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Original Research

Characterization of a Novel NDM-5-Harboring Plasmid from a Carbapenem-Resistant Escherichia coli Isolate from China

, , , , , , , , , & show all
Pages 3929-3935 | Published online: 02 Nov 2020

Figures & data

Table 1 Antibiotic Susceptibilities of E. coli ECO2947 and the E. coli J53 Transconjugants

Figure 1 S1-PFGE pattern for strain ECO2947 and southern blotting for the blaNDM-5 gene. Lanes: Marker, Salmonella serotype Braenderup strain H9812 as the size standard; 1, PFGE result for S1-digested plasmid DNA of strain ECO2947; 2, Southern blotting with the probe specific to blaNDM-5; 3, PFGE patterns for S1-digested plasmid DNA of E. coli transconjugants J53.

Figure 1 S1-PFGE pattern for strain ECO2947 and southern blotting for the blaNDM-5 gene. Lanes: Marker, Salmonella serotype Braenderup strain H9812 as the size standard; 1, PFGE result for S1-digested plasmid DNA of strain ECO2947; 2, Southern blotting with the probe specific to blaNDM-5; 3, PFGE patterns for S1-digested plasmid DNA of E. coli transconjugants J53.

Figure 2 Plasmid analysis of p2947-NDM5. (A) Genetic structure comparison of p2947-NDM5, CP033629.1), MK191836.1) and MG825370.1). (B) Comparative analysis of MDR regions. (C) Comparative analysis of the genetic contexts of blaNDM-5 in plasmids reported in this study and previously described.

Figure 2 Plasmid analysis of p2947-NDM5. (A) Genetic structure comparison of p2947-NDM5, CP033629.1), MK191836.1) and MG825370.1). (B) Comparative analysis of MDR regions. (C) Comparative analysis of the genetic contexts of blaNDM-5 in plasmids reported in this study and previously described.