Characterization of a Novel NDM-5-Harboring Plasmid from a Carbapenem-Resistant Escherichia coli Isolate from China

Figures & data

Table 1 Antibiotic Susceptibilities of E. coli ECO2947 and the E. coli J53 Transconjugants

Antimicrobial	MIC (μg/mL)
	ECO2947	J53 (The Transconjugant)	J53
Ampicillin	≥32	≥32	8
Sulbactam-Ampicillin	≥32	≥32	4
Aztreonam	≤1	≤1	≤1
Furadantin	32	≤16	≤16
Ciprofloxacin	≤0.25	≤0.25	≤0.25
Piperacillin-Tazobactam	64	64	≤4
Gentamicin	≤1	≤1	≤1
Cefepime	16	8	≤1
Ceftriaxone	≥64	≥64	≤1
Ceftazidime	≥64	≥64	≤1
Cefotetan	32	32	≤4
Cefamedin	≥64	≥64	≤4
Tobramycin	≤1	≤1	≤1
Imipenem	≥16	≥16	≤1
Amikacin	≤2	≤2	≤2
Levofloxacin	1	≤0.25	≤0.25

Figure 1 S1-PFGE pattern for strain ECO2947 and southern blotting for the bla_NDM-5 gene. Lanes: Marker, Salmonella serotype Braenderup strain H9812 as the size standard; 1, PFGE result for S1-digested plasmid DNA of strain ECO2947; 2, Southern blotting with the probe specific to bla_NDM-5; 3, PFGE patterns for S1-digested plasmid DNA of E. coli transconjugants J53.

Figure 2 Plasmid analysis of p2947-NDM5. (A) Genetic structure comparison of p2947-NDM5, CP033629.1), MK191836.1) and MG825370.1). (B) Comparative analysis of MDR regions. (C) Comparative analysis of the genetic contexts of bla_NDM-5 in plasmids reported in this study and previously described.