Figures & data
Table 1 MIC Values of Cordycepin and Fluconazole
Figure 2 Effects of cordycepin on biofilms of C. albicans. (A) Inhibitory effects on biofilm formation; (B) Eradicating effects on mature biofilms. Error bars represent the standard deviations, and different letters represent statistical differences among bars (n = 3, P < 0.05).
![Figure 2 Effects of cordycepin on biofilms of C. albicans. (A) Inhibitory effects on biofilm formation; (B) Eradicating effects on mature biofilms. Error bars represent the standard deviations, and different letters represent statistical differences among bars (n = 3, P < 0.05).](/cms/asset/0f413b5b-54c7-49b1-a70f-f215807c8a37/didr_a_285690_f0002_b.jpg)
Figure 3 CLSM images of C. albicans biofilm treated with different concentrations of cordycepin. (A), (C) and (E) show the effects of 0, 0.031, and 0.125 mg/mL of cordycepin on the formation of biofilms and live/dead cells ratio. (B), (D) and (F) show the fluorescence intensities of live (green)/dead (red) cells in biofilms treated with 0, 0.031, and 0.125 mg/mL of cordycepin, respectively. Viable cells appear green due to FDA staining, and cells with damaged membranes appear red due to PI staining.
![Figure 3 CLSM images of C. albicans biofilm treated with different concentrations of cordycepin. (A), (C) and (E) show the effects of 0, 0.031, and 0.125 mg/mL of cordycepin on the formation of biofilms and live/dead cells ratio. (B), (D) and (F) show the fluorescence intensities of live (green)/dead (red) cells in biofilms treated with 0, 0.031, and 0.125 mg/mL of cordycepin, respectively. Viable cells appear green due to FDA staining, and cells with damaged membranes appear red due to PI staining.](/cms/asset/9ccdb36c-9d0e-41f9-ae3d-9d52d119e7ff/didr_a_285690_f0003_c.jpg)
Figure 4 Changes of mature biofilms and live/dead cells before and after cordycepin treatment. (A), (C) and (E) show the effects of 0, 0.125, and 0.5 mg/mL of cordycepin on the eradication of mature biofilms and live/dead cells. (B), (D) and (F) show the fluorescence intensities of live (green)/dead (red) cells in biofilms treated with 0, 0.125, and 0.5 mg/mL of cordycepin, respectively. Viable cells appear green due to FDA staining, and cells with damaged membranes appear red due to PI staining.
![Figure 4 Changes of mature biofilms and live/dead cells before and after cordycepin treatment. (A), (C) and (E) show the effects of 0, 0.125, and 0.5 mg/mL of cordycepin on the eradication of mature biofilms and live/dead cells. (B), (D) and (F) show the fluorescence intensities of live (green)/dead (red) cells in biofilms treated with 0, 0.125, and 0.5 mg/mL of cordycepin, respectively. Viable cells appear green due to FDA staining, and cells with damaged membranes appear red due to PI staining.](/cms/asset/d022b9fe-eea8-4cca-9f80-ca17c87e4494/didr_a_285690_f0004_c.jpg)
Figure 5 Effects of cordycepin on the metabolic activity of C. albicans biofilm. (A) Effects on biofilm formation; (B) Effects on mature biofilms. Error bars represent the standard deviations, and different letters represent statistical differences among bars (n = 3, P < 0.05).
![Figure 5 Effects of cordycepin on the metabolic activity of C. albicans biofilm. (A) Effects on biofilm formation; (B) Effects on mature biofilms. Error bars represent the standard deviations, and different letters represent statistical differences among bars (n = 3, P < 0.05).](/cms/asset/6f3644e8-6aa9-4bb8-b13f-152f3886444f/didr_a_285690_f0005_b.jpg)
Figure 6 Effects of cordycepin on EPS secretion of C. albicans biofilm. (A) Effects on EPS secretion in biofilm formation; (B) Effects on EPS secretion in mature biofilms. Error bars represent the standard deviations, and different letters represent statistical differences among bars (n =3, P < 0.05).
![Figure 6 Effects of cordycepin on EPS secretion of C. albicans biofilm. (A) Effects on EPS secretion in biofilm formation; (B) Effects on EPS secretion in mature biofilms. Error bars represent the standard deviations, and different letters represent statistical differences among bars (n =3, P < 0.05).](/cms/asset/ab5a335a-75c7-47f5-92b8-fb49702363b4/didr_a_285690_f0006_b.jpg)
Figure 7 Distribution of polysaccharides and dead cells in cordycepin-treated biofilms. (A), (C) and (E) show the distribution of EPS (green) and dead cells (red) in biofilms treated with 0, 0.031, and 0.125 mg/mL of cordycepin. (B), (D) and (F) show the distribution of EPS (green) and dead cells (red) in mature biofilms treated with 0, 0.125, and 0.5 mg/mL of cordycepin.
![Figure 7 Distribution of polysaccharides and dead cells in cordycepin-treated biofilms. (A), (C) and (E) show the distribution of EPS (green) and dead cells (red) in biofilms treated with 0, 0.031, and 0.125 mg/mL of cordycepin. (B), (D) and (F) show the distribution of EPS (green) and dead cells (red) in mature biofilms treated with 0, 0.125, and 0.5 mg/mL of cordycepin.](/cms/asset/d37bb5cb-4685-40b7-8856-4d13f3a5a01b/didr_a_285690_f0007_c.jpg)