Prevalence of Aminoglycoside Resistance and Aminoglycoside Modifying Enzymes in Acinetobacter baumannii Among Intensive Care Unit Patients, Ismailia, Egypt

Figures & data

Table 1 Primers Used in This Study⁵

Primer	Primer Sequence	Product Size	Annealing Temperature
aacC1 F	ATG GGC ATC ATT CGC ACA TGT AGG	456 bp	52
aacC1 R	TTA GGT GGC GGT ACT TGG GTC
aadB F	ATG GAC ACA ACG CAG GTC GC	534 bp	55
aadB R	TTA GGC CGC ATA TCG CGA CC
aadA1 F	ATG AGG GAA GCG GTG ATC G	792 bp	52
aadA1 R	TTA TTT GCC GAC TAC CTT GGT G
aphA6 F	ATG GAA TTG CCC AAT ATT ATT C	797 bp	55
aphA6 R	TCA ATT CAA TTC ATC AAG TTT TA

Table 2 Antimicrobial Resistance Pattern of A. baumannii Against Different Antibiotics (N=52)

Antibiotics	Resistance No. (%)	Intermediate No. (%)	Sensitive No. (%)
Ampicillin/sulbactam	42 (80.8%)	0 (0.0%)	10 (19.2%)
Cefazoline	52 (100%)	0 (0.0%)	0 (0.0%)
Ceftriaxone	39 (75%)	2 (3.8%)	11 (21.1%)
Cefepime	41 (78.8%)	0 (0.0%)	11 (21.1%)
Imipenem	36 (69.2%)	0 (0.0%)	16 (30.7%)
Meropenem	36 (69.2%)	0 (0.0%)	16 (30.7%)
Amikacin	5 (9.6%)	0 (0.0%)	47 (90.4%)
Gentamicin	27 (51.9%)	0 (0.0%)	15 (28.8%)
Tobramycin	35 (67.3%)	0 (0.0%)	17 (32.7%)
Ciprofloxacin	39 (75%)	0 (0.0%)	13 (25%)
Tigecycline	0 (0.0%)	0 (0.0%)	52 (100%)
Trimethoprim/sulphamethoxazole	19 (36.5%)	0 (0.0%)	33 (63.5%)

Figure 1 Amplification of aaaC1 gene in MDR A. baumannii by PCR; Lane (L) shows 100-bp molecular size ladder, lane (P) is the positive control, lanes 1,5,6,7,9,10,11 are the positive samples carrying aaaC1 gene (456 bp). Lanes 2,3,4,8 are negative samples. Lane (12) is the negative control.

Figure 2 Amplification of aphA6 gene in MDR A. baumannii by PCR; Lane (L) shows 100-bp molecular size ladder, lane (P) is the positive control, lanes 1–11 are the positive samples carrying aphA6 gene (797 bp). Lane (N) is the negative control.

Figure 3 Amplification of aadA1 gene in MDR A. baumannii by PCR; Lane (L) shows 100-bp molecular size ladder. Lane (N) is the negative control. Lanes 3–6 are the positive samples carrying aadA1 gene (792 bp). Lanes 1.2 are negative samples. Lane (P) is the positive control.

Table 3 Distribution of AME Resistance Genes According to the Clinical Isolates

Gene	Endotracheal Aspirate No. (%)	Urine No. (%)	Sputum No. (%)	Blood No. (%)	Pus No. (%)
aacC1 (N=14)	12 (54.6%)	2 (33.3%)	0 (0%)	0 (0%)	0 (0%)
aphA6 (n=11)	8 (36.4%)	0 (0%)	2 (50%)	1 (50%)	0 (0%)
aadA1 (n=5)	1 (4.5%)	2 (33.3%)	2 (50%)	0 (0%)	0 (0%)
No genes (n=5)	1 (4.5%)	2 (33.3%)	0 (0%)	1 (50%)	1 (100%)
Total	22 (100%)	6 (100%)	4 (100%)	2 (100%)	1 (100%)

Aliakbarzade K, Farajnia S, Karimi Nik A, Zarei F, Tanomand A. Prevalence of aminoglycoside resistance genes in Acinetobacter baumannii isolates. Jundishapur J Microbiol. 2014;7(10):e11924. doi:10.5812/jjm.1192425632323

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