Investigation of One Familial Cluster of COVID-19 in Taiwan: Differentiation of Genetic Variation Among Isolates and Implications for Epidemiological Investigation and Surveillance by Genomic Assay

Figures & data

Table 1 Clinical and Laboratory Characteristics of the Familial Cluster Infection Cases

Variables	Case 14	Case 15	Case 17	Case 18
Age	53	53	24	22
Gender	Male	Female	Male	Male
Laboratory findings
White-cell count (per µL)	5030	4200	6940	5880
Hemoglobin (g/dl)	15.5	12.1	16.3	15.9
Platelet (per µL)	194,000	187,000	282,000	277,000
Differential count (%)
Neutrophils	77.7	65.3	60.8	65.4
Lymphocytes	15.9	24.5	33.9	26.9
Sodium (mmol/L)	137	134	141	140
Creatinine (mg/dl)	0.9	0.7	0.9	0.9
AST (U/L)	48	18	19	16
ALT (U/L)	46	8	25	15
C-reactive protein (mg/l)	8.60	1.37	<0.10	<0.10
Influenza screen (A &B)	Negative	Negative	Negative	Negative
SARS-Cov-2 RT-PCR	Positive	Positive	Positive	Positive
Ct-values	33	24	26	27
Signs and symptoms
Cough	Yes	Yes	Yes	No
Sore throat	Yes	Yes	Yes	No
Dyspnea	Yes	No	No	No
Fever	Yes	Yes	Yes	No
Chest radiograph	Abnormal	Abnormal	Normal	Normal

Abbreviations: AST, aspartate aminotransferase; ALT, alanine aminotransferase.

Figure 1 Genogram of family members infected with COVID-19 who returned to Taiwan from Italy, including their clinical courses.

Figure 2 Estimating TSGH strains using TimeTree with MEGA X. Strain names: Wuhan, severe acute respiratory syndrome coronavirus 2 isolate Wuhan-Hu-1; TSGH, this study. Numbers associated with strain names are GISAID Accession IDs.

Abbreviations: Eg, Egypt; It, Italy; US, United States of America.

Figure 3 Phylogenetic tree with 75 viral genome strains linking the four familial sequences.

Figure 4 Novel nucleotide substitutions found via whole-genome analysis of the asymptomatic case, Case 18.