Investigations on Acinetophage, QAB 3.4, Targeting Extensively Drug-Resistant Acinetobacter baumannii Isolates

Figures & data

Table 1 Relative PFU/mL (EOP) and Resistance Frequency of QAB 3.4 in Clinical Strains

Sr. No	Source	Sensitive Antibiotics	EOP	RRF	Sr. No	Source	Sensitive Antibiotics	EOP	RRF
1	Pus	Mino, Pb, Dox, Gen	1.2	0.8	52	Fluid	Dox, Mino, Pb	1.1	0.9
2	Blood	Mino, Pb, Dox, Gen	1.1	0.8	53	NBL	Dox, Mino, Pb	1.1	0.8
3	NBL	Mino, Pb, Dox, Gen	1.1	0.9	54	Tissue	Dox, Mino, Pb, Tgc	1.1	1
4	Blood	Pb, Mino	0.8	1	55	NBL	Gen, Tgc, Pb, Mino	0.9	1
5	Pus swab	Pb, Cot	0.2	1	56	Pus swab	Pb	0.8	1.1
6	Pus swab	Fep, Dox, Tgc, Pb, Amk, Mino	0.2	0.8	57	NBL	Pb	0.5	0.9
7	Sputum	Dox, Mino, Pb	0.6	0.9	58	EB	Pb	0.5	1.2
8	NBL	Gen, Imp, Mero, Pb, Dox, Mino	0.7	1.1	59	Tip	Pb, Tgc, Mino	0.7	1
9	Blood	Cip, Imp, Mero, Pb, Tgc, Amk, Gen, Min, Dox, Cot	0.4	1.2	60	Fluid	Pb, Dox, Mino	0.6	1.2
10	CVP tip	Pb	0.7	1.2	61	Fluid	Pb, Dox, Mino	0.6	1.3
11	Sputum	Dox, Mino, Pb	0.7	1.2	62	Sputum	Dox, Imp, Mero, Gen, Cip, Cot, Mino, Pb, Tzp, Tgc, Fep, Aug	0.4	0.8
12	Pus swab	Pb	0.9	1.3	63	Tissue	Dox, Imp, Mero, Gen, Cip, Cot, Mino, Pb, Tzp, Tgc, Fep, Aug	0.8	1.2
13	Pus	Gen, Ami, Cot, Imp, Mero	0.4	1.1	64	Blood	Tgc, Dox, Pb	0.8	1.1
14	Blood	Mino, Pb	0.5	1	65	Tissue	Pb, Tgc	0.9	1
15	NBL	Ami, Dox, Pb, Mino	0.6	0.9	66	Pus	Pb, Mino	1.1	1.1
16	Fluid	Ami, Dox, Pb, Mino	0.7	0.9	67	NBL	Dox, Mino, Pb	1	1
17	NBL	Ami, Dox, Pb, Mino	0.6	0.8	68	Blood	Dox, Mino, Pb	1.2	0.8
18	Pus swab	Pb, Tgc	0.7	0.9	69	Tissue	Dox, Mino, Pb	1.1	1.2
19	NBL	Tgc, Pb, Mino	1.2	1	70	Tip	Pb	1.2	1.3
20	NBL	Tgc, Pb, Mino, Dox	0.9	0.9	71	NBL	Pb, Tgc	1.2	0.8
21	Tip C/S	Pb	0.9	0.8	72	Tissue	Pb, Tgc	1.2	1.1
22	Bile	Tgc, Pb, Mino, Gen, Ami, Cip, Cot, Tzp	0.5	0.9	73	Pl Fluid	Dox, Mino, Pb	1.2	0.8
23	NBL	Dox, Pb, Mino	0.5	1	74	Tip	Dox, Mino, Pb	1.4	0.9
24	Urine	Cot, Imp, Mino	0.4	1	75	Tissue	Pb, Amk	1.1	1
25	Pus	Pb	0.8	0.9	76	Tissue	NONE	0.3	1.3
26	Sputum	Pb	0.9	0.8	77	Tissue	Pb	1.2	1.1
27	Pus swab	Cot, Pb, Mino	0.4	0.8	78	Pus swab	Pb	0.8	0.9
28	Fluid	Dox, Tgc, Pb, Mino, Gen, Cip, Tzp, Ami, Cot	0.4	0.8	79	Pus swab	Pb	1.4	0.9
29	Pus swab	Pb	1.1	0.9	80	Pus swab	Pb	1.2	1.3
30	NBL	Pb	1.1	0.8	81	NBL	Pb	1.3	0.8
31	NBL	Dox, Pb, Mino	1.2	1	82	CVP tip	Dox, Mino, Pb, Tgc	0.8	1.2
32	Pus	Dox, Pb, Mino, Gen	0.8	1.1	83	Pus	Dox, Mino, Pb, Gen	0.7	1.2
33	Pus swab	Gen, Dox, Cot, Pb, Mino	0.8	1.1	84	EBW	Tgc, Pb, Mino	0.7	0.8
34	Pus	Pb	0.8	1.2	85	EBW	Pb, Mino, Dox, Cot, Tgc	0.6	0.9
35	Pus	Gen, Cot, Dox, Pb	0.9	1.3	86	Pus swab	Dox, Mino, Pb	0.7	1.1
36	EBW	Pb, Dox, Mino	0.8	1.3	87	Pus swab	Gen, Ami, Dox, Pb, Mino, Tgc	0.7	1.3
37	Tissue	Gen, Cot, Dox Pb, Mino, Tgc	0.6	1.2	88	Pus swab	Tgc, Pb	0.9	1.3
38	Pus C/S	Pb	1.3	1.3	89	Pus swab	Dox, Mino	0.9	1.1
39	Tissue	Mino, Cot, Pb	1.2	1.2	90	NBL	Gen, Dox, Pb, Mino	1	1
40	Tip	Gen, Pb	1	1	91	Pus swab	Dox, Mino, Pb	1	1.2
41	NBL	Pb	1.1	1	92	NBL	Pb	1.1	0.9
42	Pus swab	Pb	1	1	93	NBL	Pb	0.7	1
43	NBL	Tgc	0.2	0.9	94	NBL	Pb, Mino	0.8	1.3
44	CSF	Pb	0.7	1.1	95	Tissue	Pb	1.1	1.3
45	Tissue	Dox, Pb, Mino	0.6	0.9	96	Pus swab	Dox, Mino, Pb, Gen	1.1	1.1
46	Pus swab	Pb	1.1	0.8	97	Tissue	Mino, Pb	1	0.9
47	Tissue	Gen, Cip, Cef, Tzp, Cot, Ami, Imp, Mero, Pb, Mino	0.3	0.8	98	Sputum	Gen, Mino, Pb, Dox	0.7	0.9
48	NBL	Cot, Dox	0.6	0.9	99	NBL	Gen, Mino, Pb, Dox	0.9	1
49	NBL	Dox, Pb	0.7	0.9	100	NBL	Pb, Mino	0.9	1
50	Pus	Pb	0.8	0.8	101	NBL	Pb, Dox, Mino	1.3	1.3

Abbreviations: Mino, Minocycline; Pb, Polymyxin B; Dox, Doxycycline; Gen, Gentamicin; Cot, Sulfamethoxazole; Fep, Cefepime; Tgc, Tigecycline; Amk, Amikacin; Cip, Ciprofloxacin; Imp, Imipenem; Mero, Meropenem; Tzp, Piperacillin - Tazobactam; Aug, Amoxycillin - Clavulanic Acid.

Figure 1 Phage-Bacteria co-culture assays. A: Bacterial growth curves at various MOI. B: Bacterial growth after 48 h of phage-bacteria co-culture. C: Phage stability test at various storage temperatures.

Table 2 Colony Forming Units (CFU/µL) of Acinetobacter baumannii from Phenol-, Saline- and Phage-Exposed Surfaces

Surface	Phenol	Phage	Saline
Lab Bench	4	12	87
Top of Incubator	3	18	101
Office Table	6	15	112
Avg±SD	4.3±1.6	15±3	100±12.5