The lncRNA CCAT2 Rs6983267 G Variant Contributes to Increased Sepsis Susceptibility in a Southern Chinese Population

Figures & data

Table 1 Frequency Distribution of Selected Characteristics in Sepsis Cases and Healthy Controls

Variables	Cases (n = 474)	Controls (n = 678)			P^a
	No.	%	No.	%
Age range, month	1–180	1–168
Mean ± SD	35.04 ±34.26	35.53±29.37			0.1811
≤60	403	85.02	595	87.76
>60	71	14.98	83	12.24
Sex
Male	301	63.5	399	58.85	0.111
Female	173	36.5	279	41.15
Sepsis subtypes
Sepsis	389	82.07	NA
Septic shock	85	17.93	NA
Prognosis
Survivors	394	83.12	NA
Non-survivors	80	16.88	NA
Number of organs with dysfunction, n (%)
1–2	276	74.39	NA
3 or more	95	25.61	NA
Source of infection n (%)
Lung infection	278	58.65	NA
Brain infection	36	7.59	NA
Primary bloodstream infection	35	7.38	NA
Abdominal infection	28	5.91	NA
Respiratory infection	18	3.8	NA
Urinary tract infection	8	1.69	NA
Others	71	14.98	NA
Infection types n (%)
Gram-positive	241	50.85	NA
Gram-negative	117	24.68	NA
Mixed Gram-negative and -positive	22	4.64	NA
Fungus	18	3.8	NA
Polymicrobial	41	8.65	NA
Negative blood culture	35	7.38	NA

Notes: ^aTwo-sided χ² test for distributions between Sepsis patients cases and controls.

Table 2 Genotype Frequency Distribution of CCAT2 in Sepsis Cases and Healthy Controls

genotype	Cases (N = 474)	Controls (N =678)	P-value^a	OR (95% CI)	P-value	Adjusted OR (95% CI)	P-value^b
CCAT2/rs6983267 T>G (HWE =0.5241)
TT	135(28.48)	240(35.40)	0.0426	1.000		1.000
TG	243(51.27)	320(47.20)		1.350(1.032–1.766)	0.0287	1.311(1.016–1.743)	0.0377
GG	96(20.25)	118(17.40)		1.446(1.027–2.036)	0.0345	1.444(1.025–2.034)	0.0355
Dominant	339(71.52)	438(64.60)	0.0133	1.376(1.067–1.774)	0.0138	1.362(1.055–1.756)	0.0175
Recessive	378(79.75)	560(82.60)	0.2225	1.205(0.893–1.626)	0.2215	1.215(0.900–1.639)	0.2039

Notes: ^aχ² tests were used to determine differences in genotype distributions between the children with sepsis and the controls. ^b Adjusted for age and gender. Statistically significant values are shown in bold (P<0.05).

Abbreviations: OR, odds ratio; HWE, Hardy–Weinberg equation.

Table 3 Stratification Analysis of Susceptibility in Sepsis Patients

Variables	TT	TG/GG	P-value	OR (95% CI)	P-value	Adjusted OR (95% CI)	P-value^a
	Patients/controls
Age, months
≤60	113/209	290/386	0.0188	1.390(1.056–1.829)	0.0190	1.376(1.044–1.812)	0.0233
>60	22/31	49/52	0.4066	1.328(0.678–2.599)	0.4079	1.382(0.700–2.729)	0.3516
Sex
Male	78/135	223/264	0.0235	1.462(1.050–2.035)	0.0245	1.463(1.050–2.037)	0.0244
Female	57/105	116/174	0.3113	1.228(0.824–1.830)	0.3128	1.241(0.832–1.852)	0.2898
Sepsis subtype
Sepsis	114/240	275/438	0.0409	1.322(1.010–1.730)	0.0422	1.311(1.001–1.717)	0.0488
Septic shock	21/240	64/438	0.0448	1.670(0.995–2.802)	0.0521	1.641(0.977–2.756)	0.0612
Prognosis
Survivors	118/240	276/438	0.0671	1.282(0.981–1.674)	0.0685	1.265(0.968–1.653)	0.0852
Non-survivors	17/240	63/438	0.0088	2.031(1.162–3.549)	0.0129	2.011(1.149–3.519)	0.0144
Number of organs with dysfunction, n (%)
1–2	75/240	201/438	0.0133	1.468(1.079–1.999)	0.0146	1.445(1.060–1.969)	0.0199
3 or more	26/240	69/438	0.1168	1.454(0.902–2.344)	0.1247	1.453(0.900–2.347)	0.1265

Notes: ^aAdjusted for age and gender. Statistically significant values are shown in bold (P<0.05).

Abbreviation: OR, odds ratio.

Table 4 False Positive Report Probability Values for Associations Between the Risk of Sepsis and CCAT2 Polymorphism Genotype

Genotype/Allele	OR (95% CI)	p-value^a	Statistical power^b	Prior Probability
				0.25	0.1	0.01	0.001	0.0001
CCAT2/rs6983267 T>G
TG Vs TT	1.350(1.032–1.766)	0.0287	0.883	0.089	0.226	0.763	0.97	0.997
GG Vs TT	1.446(1.027–2.0360)	0.0345	0.735	0.123	0.297	0.823	0.979	0.998
GG/TG Vs TT	1.376(1.067–1.774)	0.0138	0.741	0.053	0.143	0.648	0.949	0.995
GG/TG Vs TT
≤60	1.390(1.056–1.829)	0.0190	0.703	0.075	0.196	0.728	0.964	0.996
Male	1.462(1.050–2.035)	0.0245	0.561	0.116	0.282	0.812	0.978	0.998
Non-survivors	2.031(1.162–3.549)	0.0129	0.156	0.198	0.426	0.891	0.988	0.999
1–2	1.468(1.079–1.999)	0.0146	0.555	0.073	0.191	0.722	0.963	0.996

Notes: ^aThe χ2 test was used to calculate the genotype frequency distributions. ^bThe statistical power was calculated using the number of observations and the OR and P values. Statistically significant values are shown in bold (P < 0.2)

Abbreviation: OR, odds ratio.

Iwashyna TJ, Cooke CR, Wunsch H, Kahn JM. Population burden of long-term survivorship after severe sepsis in older Americans. J Am Geriatr Soc. 2012;60(6):1070–1077. doi:10.1111/j.1532-5415.2012.03989.x22642542

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