Figures & data
Figure 1 S1-PFGE pattern for E. coli L889 and Southern blot analysis indicating the blaNDM-9-and mcr-1-carrying plasmids. Lane marker, Salmonella serotype Braenderup strain H9812 as a reference size standard; L889, PFGE result of S1-digested plasmid DNA of strain E. coli L889; NDM-9, Southern blotting of L889 with the probes specific to the blaNDM-9; MCR-1, Southern blotting of L889 with the probes specific to the mcr-1.
![Figure 1 S1-PFGE pattern for E. coli L889 and Southern blot analysis indicating the blaNDM-9-and mcr-1-carrying plasmids. Lane marker, Salmonella serotype Braenderup strain H9812 as a reference size standard; L889, PFGE result of S1-digested plasmid DNA of strain E. coli L889; NDM-9, Southern blotting of L889 with the probes specific to the blaNDM-9; MCR-1, Southern blotting of L889 with the probes specific to the mcr-1.](/cms/asset/e07c04c5-9170-4da1-a8ff-647f05266290/didr_a_321732_f0001_b.jpg)
Figure 2 Comparative analysis of plasmids pL889-NDM9 and pL889-MCR1 detected in E. coli L889. (A) Comparison of blaNDM-9 coding region of plasmid pL889-NDM9 with plasmid pHNTH02-1 (MG196294), pEC013 (MG545909), pNDM-T2 (MN335919), and pHNSD138-1 (MG271839). (B) Comparison of mcr-1-carrying plasmid pL889-MCR1 with plasmids pHNTH02-1 (KY693674), pHLJ179-34 (MN232213), and p5CRE51-MCR-1 (CP021176). The circular map was generated with the BLAST Ring Image Generator (http://brig.sourceforge.net/).
![Figure 2 Comparative analysis of plasmids pL889-NDM9 and pL889-MCR1 detected in E. coli L889. (A) Comparison of blaNDM-9 coding region of plasmid pL889-NDM9 with plasmid pHNTH02-1 (MG196294), pEC013 (MG545909), pNDM-T2 (MN335919), and pHNSD138-1 (MG271839). (B) Comparison of mcr-1-carrying plasmid pL889-MCR1 with plasmids pHNTH02-1 (KY693674), pHLJ179-34 (MN232213), and p5CRE51-MCR-1 (CP021176). The circular map was generated with the BLAST Ring Image Generator (http://brig.sourceforge.net/).](/cms/asset/926854ed-8960-48eb-afeb-6c87032b392b/didr_a_321732_f0002_c.jpg)
Table 1 Minimum Inhibitory Concentrations (MICs) of Tested Antibiotics for the blaNDM-9- and Mcr-1- Positive Escherichia coli ST1101 Strain and Transconjugants