Co-Occurrence of NDM-9 and MCR-1 in a Human Gut Colonized Escherichia coli ST1011

Figures & data

Figure 1 S1-PFGE pattern for E. coli L889 and Southern blot analysis indicating the bla_NDM-9-and mcr-1-carrying plasmids. Lane marker, Salmonella serotype Braenderup strain H9812 as a reference size standard; L889, PFGE result of S1-digested plasmid DNA of strain E. coli L889; NDM-9, Southern blotting of L889 with the probes specific to the bla_NDM-9; MCR-1, Southern blotting of L889 with the probes specific to the mcr-1.

Figure 2 Comparative analysis of plasmids pL889-NDM9 and pL889-MCR1 detected in E. coli L889. (A) Comparison of bla_NDM-9 coding region of plasmid pL889-NDM9 with plasmid pHNTH02-1 (MG196294), pEC013 (MG545909), pNDM-T2 (MN335919), and pHNSD138-1 (MG271839). (B) Comparison of mcr-1-carrying plasmid pL889-MCR1 with plasmids pHNTH02-1 (KY693674), pHLJ179-34 (MN232213), and p5CRE51-MCR-1 (CP021176). The circular map was generated with the BLAST Ring Image Generator (http://brig.sourceforge.net/).

Table 1 Minimum Inhibitory Concentrations (MICs) of Tested Antibiotics for the bla_NDM-9- and Mcr-1- Positive Escherichia coli ST1101 Strain and Transconjugants

Agents	E. coli L889	Transconjugant (NDM-9)	Transconjugant (MCR-1)
Amoxicillin/clavulanate	32 (R)	32 (R)	64 (R)
Piperacillin/tazobactam	>128 (R)	>128 (R)	>128 (R)
Cefotaxime	>128 (R)	>128 (R)	>128 (R)
Ceftazidime	>128 (R)	>128 (R)	>128 (R)
Cefpirome	>128 (R)	64 (R)	>128 (R)
Cefepime	128 (R)	64 (R)	128 (R)
Meropenem	4 (R)	2 (I)	2 (I)
Imipenem	8 (R)	8 (R)	4 (R)
Ertapenem	16 (R)	8 (R)	2 (S)
Aztreonam	128 (R)	0.5 (S)	128 (R)
Gentamicin	>128 (R)	1 (S)	>128 (R)
Amikacin	>128 (R)	4 (S)	>128 (R)
Levofloxacin	32 (R)	0.5 (S)	64 (R)
Ciprofloxacin	>64 (R)	0.5 (S)	>64 (R)
Trimethoprim/sulfamethoxazole	>8 (R)	<0.125 (S)	>8 (R)
Tetracycline	64 (R)	0.5 (S)	64 (R)
Fosfomycin	>512 (R)	1 (S)	>512 (R)
Nitrofurantoin	64 (I)	4 (S)	16 (S)
Tigecycline	0.125 (S)	0.25 (S)	0.125 (S)
Colistin	16 (R)	2 (S)	16 (R)