Co-occurrence of Klebsiella variicola and Klebsiella pneumoniae Both Carrying bla_KPC from a Respiratory Intensive Care Unit Patient

Figures & data

Table 1 Determination of Minimum Inhibitory Concentration (MIC) for K. variicola SKv2E and K. pneumoniae SKp2F and Their bla_KPC Transconjugants

MIC (μg/mL)	AMP	ATM	CAZ	CIP	CRO	CXM	SAM	FEP	GEN	IPM	MEM	SXT	TGC	PB
SKv2E	≥32	≥32	≥64	≥4	≥16	≥32	≥32	≥64	≥16	≥8	≥8	≥160	0.5	1
SKp2F	≥32	≥32	≥64	≥4	≥16	≥32	≥32	≥64	≥16	≥8	≥8	≥320	0.5	0.5
J53-pSKv2E-blaKPC	≥32	≥32	≥32	≥4	≥16	≥32	≥32	≥32	≥16	≥8	≥8	160	0.25	1
J53-pSKp2F-blaKPC	≥32	≥32	≥32	≥4	≥16	≥32	≥32	≥32	≥16	≥8	≥8	160	0.25	0.5
J53	1	0.125	0.25	0.125	1	0.25	0.5	0.25	0.5	0.125	0.25	4	0.125	0.5

Abbreviations: AMP, ampicillin; ATM, aztreonam; CAZ, ceftazidime; CIP, ciprofloxacin; CRO, ceftriaxone; CXM, cefuroxime; SAM, ampicillin–sulbactam; FEP, cefepime; GEN, gentamicin; IPM, imipenem; MEM, meropenem; SXT, sulfamethoxazole–trimethoprim; TGC, tigecycline; PB, polymyxin B.

Table 2 Genome Characteristics of K. variicola SKv2E and K. pneumoniae SKp2F

Isolate	SKv2E	SKp2F
Genome length (bp)	6,115,610	5,403,687
No of scaffolds	126	45
No of tRNA	79	84
No of rRNA	15	14
No of ncRNA	10	14
No of CDs	5089	5231
MLST	ST1615	ST631
Resistance genes	blaKPC-2, blaTEM-1A, blaLEN17, aadA16, arr-3, qnrB4, oqxA/B, dfrA27, sul1, tetD, fosA, qacEΔ1	blaKPC-2, blaCTX-M-3, blaTEM-1B, blaCTX-M-65, blaSHV-27, aac(6ʹ)-IIa, rmtB, arr-3, aph(3ʹ)-Ia, aadA16, qnrS1, aac(6ʹ)-Ib-cr, qnrB91, oqxA/B, mph(A), tet(A), fosA, dfrA27, two copies of qacEΔ1-sul1
Grade of human serum resistance	4	3
String testing	Non-hypermucoviscous	Non-hypermucoviscous
Mean biofilm formation (OD595)	1.93	1.65
Plasmid replicons	Col(pHAD28), Col440I, IncFIB(K), IncFII(K), IncFII(Yp), IncHI1B	IncFII(K)

Figure 1 Schematic mapping of the genetic characteristics of the resistance gene (bla_KPC-2)-carrying region in strain K. variicola SKv2E and K. pneumoniae SKp2F. The construction of the sequence comparison was performed using blast (http://blast.ncbi.nlm.nih.gov). Genes are shown as arrows, and their orientations of transcription are indicated by the arrowheads.

Figure 2 Schematic mapping of the genetic characteristics of resistance gene (arr-3, dfrA27, aadA16, qnrB)-carrying region in strain K. variicola SKv2E and K. pneumoniae SKp2F. The construction of the sequence comparison was performed using blast (http://blast.ncbi.nlm.nih.gov). Genes are shown as arrows, and their orientations of transcription are indicated by the arrowheads.