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Original Research

High Rates of Aminoglycoside Methyltransferases Associated with Metallo-Beta-Lactamases in Multidrug-Resistant and Extensively Drug-Resistant Pseudomonas aeruginosa Clinical Isolates from a Tertiary Care Hospital in Egypt

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Pages 4849-4858 | Published online: 19 Nov 2021

Figures & data

Table 1 Primers Used for the Detection of aac(6ʹ)-lb, aac(3)-lla, rmtB, rmtC, armA, rmtD, blaNDM, blaVIM, and blaIMP Genes, and Expected Product Size

Table 2 Association Between Aminoglycoside Resistance and Carbapenem Resistance Genotypes in 19 Positive Aminoglycoside Resistance Genes in P. aeruginosa Clinical Isolates

Figure 1 The 173-bp PCR amplification of rmtB gene among P. aeruginosa isolates. First lane, DNA ladder, 100-bp DNA size marker; (1) P, positive control, from 2 to 20 clinical isolates; −ve, negative control.

Figure 1 The 173-bp PCR amplification of rmtB gene among P. aeruginosa isolates. First lane, DNA ladder, 100-bp DNA size marker; (1) P, positive control, from 2 to 20 clinical isolates; −ve, negative control.

Figure 2 The 315-bp PCR amplification of armA gene among P. aeruginosa isolates. (1) P, positive control; second lane, DNA ladder, 100-bp DNA size marker; (2) and (3), clinical isolates with positive results; −ve, negative control.

Figure 2 The 315-bp PCR amplification of armA gene among P. aeruginosa isolates. (1) P, positive control; second lane, DNA ladder, 100-bp DNA size marker; (2) and (3), clinical isolates with positive results; −ve, negative control.

Figure 3 The 482-bp PCR amplification of aac(6ʹ)-lb gene among P. aeruginosa isolates. First lane, DNA ladder, 100-bp DNA size marker; (1) P, positive control; (2), (3), (4), and (5), clinical isolates; −ve, negative control.

Figure 3 The 482-bp PCR amplification of aac(6ʹ)-lb gene among P. aeruginosa isolates. First lane, DNA ladder, 100-bp DNA size marker; (1) P, positive control; (2), (3), (4), and (5), clinical isolates; −ve, negative control.

Figure 4 The 700-bp PCR amplification of blaNDM gene among P. aeruginosa isolates. First lane, DNA ladder, 100-bp DNA size marker; −ve, negative control.

Figure 4 The 700-bp PCR amplification of blaNDM gene among P. aeruginosa isolates. First lane, DNA ladder, 100-bp DNA size marker; −ve, negative control.

Figure 5 The 390-bp PCR amplification of blaVIM gene among P. aeruginosa isolates. First lane, DNA ladder, 100-bp DNA size marker; −ve, negative control.

Figure 5 The 390-bp PCR amplification of blaVIM gene among P. aeruginosa isolates. First lane, DNA ladder, 100-bp DNA size marker; −ve, negative control.