Profile of Bacteria with ARGs Among Real-World Samples from ICU Admission Patients with Pulmonary Infection Revealed by Metagenomic NGS

Figures & data

Table 1 Demographic Characteristics of ICU Admission Patients with Pulmonary Infection

	N (%)	Bacteria with ARGs Detected		P
		Yes	No
Gender
Male	312 (71.89%)	144 (46.15%)	168 (53.85%)
Female	122 (28.11%)	48 (39.34%)	74 (60.66%)	0.237
Age	61.03±15.18
<18	22 (5.07%)	9 (40.91%)	13 (59.09%)
18–30	15 (3.46%)	4 (26.67%)	11 (73.34%)
31–60	141 (32.49%)	63 (44.68%)	78 (55.32%)
61–96	256 (58.99%)	116 (45.31%)	140 (54.69%)	0.5504
Antibiotic history
Yes	376 (86.64%)	167 (44.41%)	209 (55.59%)
No	58 (13.36%)	25 (43.10%)	33 (56.90%)	0.8878
Department
GICU	306 (70.51%)	148 (48.37%)	158 (51.63%)
PICU	16 (3.69%)	7 (43.75%)	9 (56.25%)
RICU	89 (20.51%)	28 (31.46%)	61 (68.54%)
SICU	7 (1.61%)	5 (71.43%)	2 (28.57%)
EICU	16 (3.69%)	4 (25.00%)	12 (75.00%)	0.014
Comorbidities
None	172 (39.63%)	72 (41.86%)	100 (58.14%)
Respiratory disorders	36 (8.29%)	13 (36.12%)	23 (63.88%)
Malignancies	51 (11.75%)	19 (37.26%)	32 (62.74%)
Brain disease	37 (8.53%)	24 (64.87%)	13 (35.13%)
Cardiovascular disease	37 (8.53%)	17 (45.95%)	20 (54.05%)
Renal+ liver disease	21 (4.84%)	8 (38.10%)	13 (61.90%)
Diabetes/diabetes +others	20 (4.61%)	10 (50.0%)	10 (50.0%)
Autoimmune diseases	18 (4.15%)	8 (44.45%)	10 (55.55%)
Trauma	8 (1.84%)	5 (62.50%)	3 (37.50%)
Organ transplantation	11 (2.53%)	8(72.73%)	3(27.27%)
Others	23 (5.30%)	8(34.79%)	15(65.21%)	0.1351

Figure 1 Spectrum and absolute number of bacteria with ARGs detected in samples from ICU admission patients with pulmonary infection. (A) Spectrum and absolute number of bacteria with ARGs detected in samples from different patients groups. (B) Spectrum and absolute number of bacteria with ARGs detected in different types of samples.

Figure 2 Long tail of bacteria with ARGs identified by mNGS in clinical samples from ICU admission patients with pulmonary infection.

Figure 3 Comparison of bacteria with ARGs detected by mNGS from different types of samples. (A) Bacteria with ARGs detected in different types of samples. (B) Bacteria with ARGs detected in paired-sample from same patients. ^#Indicate no statistical significance; *Indicate statistical significance.

Abbreviation: PF, pleural fluid.