Deterioration of Cycloserine in Drug Susceptibility Testing of Mycobacterium

Figures & data

Figure 1 The cycloserine DST of M. hassiacum 95085. The cycloserine solutions had been previously incubated at 37 °C for 1 to 29 days. According to Alamar Blue staining, which is an indicator of the MIC, the results can be divided into three stages.

Figure 2 The chromatograms (A) and mass spectrums from LC-MS (B) of two cycloserine solutions that had been incubated at 37 °C for 1 and 29 days, respectively.

Table 1 The Peak Area and Percentage of Degradation of Cycloserine in HPLC

Days of Incubation at 37°C	Area	Percentage of Degradation (%)
1	5121812	–
2	5117030	0.09
3	5111988	0.19
4	5064697	1.12
5	5051009	1.38
6	5010276	2.18
7	4950229	3.35
8	4946033	3.43
9	4845164	5.40
15	4686213	8.50
20	4571746	10.74
29	4377712	14.53

Figure 3 The degradation ratio map of cycloserine in MH medium.