Circulation of Dengue Virus Serotype 2 in Humans and Mosquitoes During an Outbreak in El Quseir City, Egypt

Figures & data

Figure 1 Map of Egypt displaying the location of the Red Sea Governorate and its cities. Site of the study is Quseir City adapted from https://freesvg.org/1546672123.¹⁸

Table 1 The Five Oligonucleotide Primers Used in Multiplex PCR for Identification of the DENV Serotype in the Present Study

Primer	Primer Sequence
D1	5ʹTCAATATGCTGAAACGCGCGAGAAACCG-3’
TS1	5’-CGTCTCAGTGATCCGGGGG-3’
TS2	5’-CGCCACAAGGGCCATGAACAG-3’
TS3	5’-TAACATCATCATGAGACAGAGC-3’
TS4	5’-CTCTGTTGTCTTAAACAAGAGA −3’

Table 2 The Oligonucleotide Primer Set for Each Virus Serotype and Expected Product Size

Virus Serotype	Primers Used	Expected Weight
DENG-1	D1 + TS1	482 bp
DENG-2	D1 +TS2	119 bp
DENG-3	D1 +TS3	290 bp
DENG-4	D1 +TS4	389 bp

Table 3 Epidemiological Data and Clinical Manifestations of Patients Participating in the Present Study

Variables	Number and Percentage (%)
N	144
Age (years) (Mean ± SD)	41.4 ± 13.74.
Gender
Male	97 (67.4)
Female	47 (32.6)
Presenting Clinical Manifestations	N %
Fever	144 (100)
Headache	144 (100)
Arthralgia	132 (91.7)
Myalgia	129 (89.6)
Bleeding tendency	25 (17.4)

Table 4 Results of ELISA/IgM Examination of Patients Samples Layered by Age and Gender

Gender/Age		ELISA/IgM
		Positive	Negative
		No. (%)	No. (%)
Male	<18	28 (28.8)	8 (17)
	>18	35 (36.1)	12 (25.5)
Female	<18	20 (20.1)	11 (23.4)
	>18	14 (14.4)	16 (34)
Total		97	47

Table 5 Results of Multiplex PCR Examination of ELISA/IgM Positive Samples Layered by Age and Gender

Gender/Age		PCR
		Positive	Negative
		No. (%)	No. (%)
Male	<18	15 (23.8)	4 (11.8)
	>18	21 (33.3)	9 (26.5)
Female	<18	17 (27)	7 (20.5)
	>18	11 (17.4)	14 (41)
Total		63	34

Figure 2 Agarose gel electrophoretic analysis of DENG samples amplified by One-Step Multiplex RT-PCR. (A) Human samples; amplified products of clinical samples, positive samples showing 119 bp (DENV-2). (B) Amplified products of Adult female Aedes aegypti, positive samples showing 119 bp (DENV-2). DNA ladder 100 bp.

Table 6 Species of Mosquitoes Tested for Arboviruses, Collected Between October 2017 and December 2017, Quseir City, Red Sea Governorate, Egypt

Mosquitoes	Male	Female	Total
Culex pipiens	39	31	70
Aedes aegypti	17	12	29
Anopheles multicolor	19	22	41
Anopheles sergentii	23	30	53

Free*SVG [homepage on the Internet].Egypt, administrative divisions map. 2019-2020. Available from https://freesvg.org/1546672123. Accessed May 23, 2022.

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