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ORIGINAL RESEARCH

Genetic Characterization of blaNDM-1-Carrying Citrobacter portucalensis Sequence Type 328 and Citrobacter freundii Sequence Type 98

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Pages 2235-2242 | Published online: 28 Apr 2022

Figures & data

Table 1 Antimicrobial Susceptibility Profiles for Citrobacter spp. Strains

Table 2 Overall Features of the Citrobacter spp. Genomes

Figure 1 Comparison of blaNDM-1-carrying plasmids. (A) Comparison of pNDM-Cf7303 (CP092467) with its maximum identity of sequences of pCFR17-1 (NC_ CP035277) and pHNTS45-1(NZ_MK167988). (B) Comparison of pNDM-Cf7308 (CP092465) with pNDM-BTR (KF534788) and pNDM-CWH001 (NZ_CM008471). Open reading frames are denoted by block arrows and colored based on gene function. Insertion sequence (IS) elements are shown in yellow, antibiotics resistant genes in blue, and others in grey. Shading regions denote regions of homology (> 95% nucleotide identity).

Figure 1 Comparison of blaNDM-1-carrying plasmids. (A) Comparison of pNDM-Cf7303 (CP092467) with its maximum identity of sequences of pCFR17-1 (NC_ CP035277) and pHNTS45-1(NZ_MK167988). (B) Comparison of pNDM-Cf7308 (CP092465) with pNDM-BTR (KF534788) and pNDM-CWH001 (NZ_CM008471). Open reading frames are denoted by block arrows and colored based on gene function. Insertion sequence (IS) elements are shown in yellow, antibiotics resistant genes in blue, and others in grey. Shading regions denote regions of homology (> 95% nucleotide identity).

Figure 2 A whole-genome sequence phylogenetic tree calculated from Cf7308, Cf7313, and 34 available genomes of C. freundii producing less common beta-lactamase such as OXA beta-lactamases, KPC, VIM, and NDM. C. freundii strain B38 (GenBank accession number CP016762) was used as the reference genome for comparison. NA of MLST indicates not be assigned a matched ST when querying the multilocus sequence typing (MLST) database of Citrobacter spp.

Figure 2 A whole-genome sequence phylogenetic tree calculated from Cf7308, Cf7313, and 34 available genomes of C. freundii producing less common beta-lactamase such as OXA beta-lactamases, KPC, VIM, and NDM. C. freundii strain B38 (GenBank accession number CP016762) was used as the reference genome for comparison. NA of MLST indicates not be assigned a matched ST when querying the multilocus sequence typing (MLST) database of Citrobacter spp.