Cluster of Differentiation 24 Polymorphism Has No Significant Association with Chronic Hepatitis B Virus Infection in the Chinese Han Population: A Family-Based Association Study

Figures & data

Figure 1 Genotyping of the CD24 gene polymorphism P170 by BstXI RFLP. The 1000 bp ladder was run as a standard. PCR fragments with TT underwent digestion to yield 2 fragments (275 and 129 bp, respectively); those with CT yielded 3 fragments (275, 129 and 404 bp, respectively), while CC-containing fragments were undigested (404 bp).

Table 1 Structures of CHB Families

	One Child	Two Children	Three Children	Four Children	Five Children	Total
Two parents	58	26	7	3	2	96
One parent	0	57	24	6	0	87
No parent	0	0	34	12	1	47
Total	58	83	65	21	3	230

Notes: A total of 230 core families with 796 in-family members were selected; these included 279 parents and 517 children, and 412 males and 384 females.

Table 2 Associations of the P170 Genotype with Chronic HBV Infection by Family-Based Association Test

Marker	Genotype	Frequency	Family^a	S	E (S)	Var (S)	Z^b	P
P170T/C	C/C	0.393	62	38.000	40.184	17.494	−0.522	0.601
	C/T	0.504	71	54.000	50.573	20.827	0.751	0.452
	T/T	0.103	28	15.000	16.243	8.446	−0.428	0.668

Notes: ^aNumber of nuclear families informative for a given allele. S, test statistic; E (S) and Var (S) represent the test statistic’s expected value and variance, respectively. ^bA negative Z value suggests that the frequency of the transmitted genotype is negatively associated with susceptibility to HBV.

Table 3 Genotypes distribution of CHB families

	Genotypes			Total
	CC	TT	T/C
Infected	185	39	254	478
Non-infected	132	45	141	318
Total	317	84	395	796

Note: Out of all the study subjects, 478 were infected and 318 were non-infected, including a total of 317 with CC, 84 with TT, and 395 with CT.

Table 4 TDT of Association with HBV-Affection Status in 478 Trios Selected from the HBV Nuclear Families

SNP	Frequency	Allele	Transmitted	Not	χ^2	P
P170C/T	0.3546	T	29	31	0.065	0.897
	0.6454	C	31	29

Note: TDT analysis revealing T and C allele (P170) transmission, respectively, to chronic HBV-infected offspring.

Table 5 Associations of P170 Polymorphisms with Chronic HBV Infection Assessed by Family-Based Association Test (Single-Marker FBAT Analysis)-in the Additive, Dominant and Recessive Models

Allele	Additive	Model		Dominant	Model		Recessive	Model
	Families^a	Z^b	P	Families	Z	P	Families	Z	P
T	72	0.169	0.866	62	0.522	0.602	28	−0.428	0.669
C	72	−0.169	0.866	28	0.428	0.669	62	−0.522	0.602

Notes: ^aInformative families might have two heterozygote parents or many offspring. With <10 informative families, the test could not be performed. ^bA negative Z value suggests that the frequency of the transmitted allele is negatively associated with susceptibility to HBV.