Bioprospecting by Phage Display of Mimetic Peptides of Chlamydia trachomatis for Use in Laboratory Diagnosis

Figures & data

Figure 1 Comparison of the reactivity of clones identified by phage display between the sera of patients who were positive and negative for C. trachomatis. (A) Phage ELISA with the clones selected by biopanning and sequenced. The clones marked with “*” were selected for use in the ELISA optimization test. Evaluation of reactivity by phage ELISA of individual samples with clones G1 (B), H5 (C), H7 (D) and C6 (E).

Table 1 Amino Acid Sequences of the Clones That Showed the Best Performance in the Phage ELISA Tests

Clone	Peptide	Alignment	Query cover	%Id	Putative target	GenBank
G1	ACMKVSPDSCGGGS	SPDSCGGGS	74%	77.7%	Cell wall-associated hydrolase domain-containing protein	CRH69585.1
		SPDS G GS
		SPDS-GSGS
H5	ACNHARTLTCGGGS	HART–LTC	71%	66.6%	Glycerol-3-phosphate acyltransferase PlsX	CRH67789.1
		HART L C
		HARTQPLVC
C6	ACYHHDSRGCGGGS	YHHD-SRGC	57%	77.7%	Transposase and inactivated derivatives	CPR81983.1
		YHHD SR C
		YHHDPSRSC
H7	ACALPSDRTCGGGS	LPSDR	64%	100%	GTP-binding protein	CRH84351.1
		LPSDR
		LPSDR

Abbreviation: Id, identification.

Figure 2 Conformational alignment of peptides from clones G1, H5, C6 and H7 in CPAF of C. trachomatis.

Figure 3 Statistical parameters of the mimetic peptides. Sensitivity, specificity, likelihood ratio (LR) and area under the curve (AUC) and ROC curve.