Diagnosis of Mycoplasma hominis Meningitis with Metagenomic Next-Generation Sequencing: A Case Report

Figures & data

Figure 1 Head and lung CT scans of the patient after admission in our hospital. (A). Head CT showing a small amount of blood, effusion, and swelling of the surrounding soft tissue in the basal ganglia after intracerebral hemorrhage surgery. (B). Lung CT showing no obvious signs of infection.

Table 1 CSF Indicators from Five Lumbar Punctures After Admission to Our Hospital

Date	Pressure (mmH2O)	White Blood cell (Count/uL)	Chloride (mmol/L)	Glucose (mmol/L)	Protein (g/L)
04/30/2021	270	810	114.5	0.28	1.32
05/05/2021	220	330	111.4	<0.28	1.95
05/12/2021	100	0	122.1	0.70	1.19
05/19/2021	70	28	121.9	1.84	0.82
05/26/2021	50	42	124.6	2.55	0.65

Abbreviation: CSF, cerebrospinal fluid.

Figure 2 mNGS analysis reveals the mapping and distribution of M. hominis reads. (A) Mapping of M. hominis reads on the genome with a coverage of 3.7%. (B) (left) Total read distribution in the CSF sample, and (right) read distribution without the human host.

Figure 3 A 399-bp target fragment mapping to M. hominis, identified by sequence-specific PCR. Lane M: DNA ladder; Lane 1: negative control; Lane 2: annealing temperature of 58 °C; Lanes 3 and 4: annealing temperature of 60 °C.