https://orcid.org/0000-0002-8838-7814
Figures & data
Figure 1 Pulmonary imaging manifestations of the patient. (A) Irregular nodules in the left upper lung. (B) Ground glass opacities and small nodules in the upper lobe of the right lung. (C) Diffuse ground glass opacities in both lungs. Lesions mentioned above were pointed by the green arrows.
Figure 2 The morphology of R. pusillus. Hyphae forms of R. pusillus in sputum stained with Gram stain (A) Original magnification X 1000) and fluorescent stain (B), Original magnification X 400). R. pusillus colony grown in 28 ℃ PDA medium for 7 days. (C) Lactophenol cotton blue (LPCB) staining of the R. pusillus colony. (D), Original magnification X 400).
Figure 3 Comparisons of the R. pusillus isolate and reference sequences. (A) All 18S rRNA reference sequences and genomes were obtained from the NCBI database. Muscle (V3.8.31) was used for multisequence comparison of 18S rRNA sequences. IQTree (V 1.6.12) was used to draw the evolutionary tree. (B) All reference genome sequences used were obtained from the NCBI database. Nucmer (V 3.1) was used to compare the whole-genome sequences, Mummer (V 3.23) was used to compare the large-fragment sequences in the genome, and FastTree (V 2.1.11) was used to draw the whole-genome phylogenetic tree after data integration. Finally, ITOL was used to visualize the figure.
Table 1 Anti-Fungal Susceptibility of the Clinical Isolate R. pusillus JY54