Impact of Alternative Growth Supplements on Antimicrobial Susceptibility Testing of Neisseria gonorrhoeae

Figures & data

Table 1 Categorical and Essential Agreements of Two Different Growth Supplements for 8 Antibiotics

	Spectinomycin		Azithromycin		Ciprofloxacin		Ceftriaxone
	Sheep blood	Hemo-globin	Sheep blood	Hemo-globin	Sheep blood	Hemo-globin	Sheep blood	Hemo-globin
Number tested	80	80	80	80	80	80	80	80
Essential agreement	80	80	76	72	78	79	72	48
Essential agreement rate	100.00%	100.00%	95.00%	90.00%	97.50%	98.75%	90.00%	60.00%
Categorical agreement	80	80	76	78	80	79	79	73
Categorical agreement rate	100.00%	100.00%	95.00%	97.50%	100.00%	98.75%	98.75%	91.25%
Very major errors	0	0	0	0	0	0	0	0
Major errors	0	0	0	0	0	1	0	0
Minor errors	0	0	4	2	0	0	1	7
Minor error rate	0.00%	0.00%	5.00%	2.50%	0.00%	0.00%	1.25%	8.75%
	Zoliflodacin		Gentamicin		Penicillin		Tetracycline
	Sheep blood	Hemo-globin	Sheep blood	Hemo-globin	Sheep blood	Hemo-globin	Sheep blood	Hemo-globin
Number tested	80	80	80	80	80	80	80	80
Essential agreement	72	31	80	80	75	65	78	73
Essential agreement rate	90.00%	38.75%	100%	100%	93.75%	81.25%	97.50%	91.25%
Categorical agreement	76	47	80	80	75	66	78	77
Categorical agreement rate	95.00%	58.75%	100%	100%	93.75%	82.50%	97.50%	96.25%
Very major errors	0	0	0	0	0	0	0	0
Major errors	0	0	0	0	0	0	0	0
Minor errors	4	33	0	0	5	14	2	3
Minor error rate	5.00%	41.25%	0.00%	0.00%	6.25%	17.50%	2.50%	3.75%

Table 2 The Percentage (%) of N. Gonorrhoeae Isolates with Different MICs (Mg/L) for Eight Antibiotics in Different Groups

MIC (mg/L)	SPE (%)				GEN (%)			PEN (%)			TET (%)
	V		S	H	V	S	H	V	S	H	V	S		H
0.25										2.50
0.5								5.00	5.00	1.30
1								11.30	15.00	12.50	7.50	5.00		3.80
2								23.80	22.50	11.30	26.30	15.00		7.50
4								21.30	22.50	21.30	61.30	75.00		51.30
8					56.30	68.80	26.30	13.80	10.00	23.80	0.00	0.00		32.50
16	20.00		13.80	5.00	43.80	31.30	73.80	0.00	0.00	2.50	0.00	0.00		0.00
32	75.00		81.30	90.00				25.00	25.00	25.00	5.00	5.00		5.00
≥64	5.00		5.00	5.00
MIC (mg/L)	ZOLI (%)				AZM (%)			CIP (%)			CRO (%)
	V	S		H	V	S	H	V	S	H	V		S	H
≤0.008											12.50		8.80	7.50
0.015											18.80		18.80	0.00
0.03	7.50							20.00	20.00	18.80	0.00		10.00	3.80
0.06	21.30	5.00						0.00	0.00	0.00	8.80		0.00	27.50
0.125	65.00	48.80		6.30	2.50			5.00	5.00	5.00	18.80		12.50	16.30
0.25	6.30	41.30		51.30	3.80	1.30	18.80	0.00	0.00	0.00	26.30		27.50	2.50
0.5		5.00		40.00	57.50	61.30	56.30	0.00	0.00	0.00	10.00		12.50	28.80
1				2.50	13.80	15.00	5.00	0.00	0.00	0.00	5.00		10.00	13.80
2					2.50	2.50	0.00	5.00	2.50	5.00
4					0.00	0.00	7.50	6.30	2.50	0.00
8					20.00	20.00	12.50	0.00	7.50	5.00
16								63.80	62.50	66.30

Abbreviations: ZOLI, zoliflodacin; CRO, ceftriaxone; PEN, penicillin; GEN, gentamicin; TET, tetracycline; SPE, spectinomycin; AZM, azithromycin; CIP, ciprofloxacin; CRO, ceftriaxone; V, Vitox; S, sheep blood; H, hemoglobin.

Figure 1 (A and B) The proportions of N. gonorrhoeae isolates with different MICs for eight antibiotics in different groups. The MIC50 (yellow-dotted line), MIC90 (red-dotted line) and number of isolates per province are shown. The block color represents the MIC value and the block length represents the percentage. The vertical coordinate represents the antibiotics of different growth supplement groups.

Abbreviations: ZOLI, zoliflodacin; CRO, ceftriaxone; PEN, penicillin; GEN, gentamicin; TET, tetracycline; SPE, spectinomycin; AZM, azithromycin; CIP, ciprofloxacin; CRO, ceftriaxone; V, Vitox; S, sheep blood; H, hemoglobin.