Genomic Characterization of Livestock-Associated Methicillin-Resistant Staphylococcus aureus ST7 Isolates from a Case of Human Bacteremia in China

Figures & data

Figure 1 Timeline of the disease. Lung CT scan at different time points were listed. Isolation of strain FJ0318 and FJ0322 were marked with triangle.

Table 1 Antimicrobial Susceptibility Testing of Strains

Antimicrobial Agent	FJ0318		FJ0322
	Diameter/MIC	Result	Diameter/MIC	Result
Cefoxitin	12	R	14	R
Gentamicin	24	S	24	S
Erythromycin	30	S	28	S
Clindamycin	24	S	22	S
Ciprofloxacin	26	S	24	S
Quinupristin-dalfopristin	28	S	26	S
Tetracycline	8	R	28	S
Linezolid	34	S	32	S
Vancomycin	0.75*	S	0.75*	S

Notes: The unit of number is millimeter or Minimum Inhibitory Concentration (MIC), R/S: resistance/ susceptibility, *Susceptibility testing for vancomycin was performed using the Etest strip.

Figure 2 Structure of pTET-ST7, the plasmid in strain FJ0318. The inner circle represents GC content. The outer circle represents ORFs. The resistance genes, replications, transposable elements, and plasmid recombination were shown beside the relevant ORFs. The plasmid sequence was aligned to pE2, pN315, SR153_pSR02 and pT8G. The relevance of circle color and plasmid was in top right corner.

Table 2 Genomic, Pathogenicity Islands and Virulence Genes in FJ0318

Island/Gene	FJ0318 (ST7)	SA268 (ST59)	LAC (ST8)	MW2 (ST1)	N315 (ST5)	COL (ST250)
νSa1	–	+	+	–	–	+
νSa2	–	–	–	–	–	–
νSa3	–	–	–	+	–	–
νSa4	–	–	–	+	+	+
νSaα	+	+	+	+	+	+
νSaβ (lukDE)	+	+	+	+	+	+
ΦSa1	–	–	_	–	–	–
ΦSa2 (containing PVL)	–	+	+	+	–	–
ΦSa3	+	–	+	+	+	–
aur, hlgA	+	+	+	+	+	+
hlgb, hlgC	+	+	+	+	+	+
hla	+	+	+	+	+	+
sak	+	–	+	+	+	+
chp	–	+	+	–	+	–
scn	+	+	+	+	+	–
lukDE	+	–	+	+	+	+
tsst	–	–	–	–	+	–
psmα1–4	+	+	+	+	+	–
psmβ1-2	+	+	+	+	+	–
Enterotoxin	sep	seb, sek, seq	sek, seq	sea, sec, sek, sel, seq	sec, seg, sei, sel, sem, sen, seo, sep, seu	seb, sek, seq

Abbreviations: +/−, presence/absence of islands or genes; aur, aureolysin; hlg, gamma-hemolysin component; hla, alpha hemolysin; sak, staphylokinase; chp, chemotaxis inhibitory protein; scn, staphylococcal complement inhibitor; tsst, toxic shock syndrome toxin-1; psm, phenol-soluble Modulins.

Figure 3 Chromosome of FJ0318 and FJ0322. The inner circle represents GC content and GC skew. The outer circle represents genomic islands and prophage. The relevance of circle color and strain was in top right corner.

Figure 4 Structure and comparative analysis of prophage page-ST7. Compared with MSSA ST7 strain 08–02300 (CP015646) and Staphylococcus aureus phage SP6 (JX274647).