Co-Existence of KPC-2, LAP-2, and CTX-M-65 in an ST1469 Multidrug-Resistant Klebsiella pneumoniae Strain in China

Figures & data

Table 1 Antimicrobial Susceptibilities of K. pneumoniae Strain SJ25 and Transconjugant SJ25-P

Antimicrobials	MIC Values (mg/L)
	SJ25	SJ25-P
Amoxicillin/clavulanate	128	128
Piperacillin/tazobactam^a	>128	2
Ceftriaxone	>128	16
Cefotaxime	>128	16
Cefepime	16	1
Ceftazidime	128	1
Ciprofloxacin	0.03	2
Levofloxacin	2	8
Imipenem	4	4
Meropenem	2	2
Ertapenem	2	2
Trimethoprim/ sulfamethoxazole	>8/152	8/152
Amikacin	>128	1
Gentamicin	>128	1
Aztreonam	64	8
Chloramphenicol	32	128
Fosfomycin	4	64
Colistin	2	1
Tigecycline	1	2

Note: ^aTazobactam at a fixed concentration of 4mg/L.Abbreviation: MIC, minimal inhibitory concentration.

Figure 1 Plasmid profiles and Southern blot-hybridization of K. pneumoniae strain SJ25. Southern blot-hybridization of S1-nuclease digested DNA using a specific probe (bla_KPC). M: XbaI digested total DNA of Salmonella enterica serotype Braenderup H9812 as a size marker.

Figure 2 Major structural features and comparison of beta-lactamase-encoding plasmids. (A) Genomic map of the bla_KPC-2 and bla_CTX-M-65 co-producing IncFII/IncR pSJ25_KPC_CTX plasmid with four closely related plasmids (CP090434.1, CP090464.1, CP101775.1, CP033956.1). (B) Circular genome alignment of pSJ25_LAP with four bla_LAP-bearing plasmids (CP029382.1, CP103318.1, CP033957.1, and CP034126.1). Open reading frames (ORFs) are indicated by arrows and colored according to their putative functions. Alignment of plasmids was performed and visualized by BLAST ring image generator (BRIG) software.