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ORIGINAL RESEARCH

Antimicrobial Resistance Patterns, Sequence Types, Virulence and Carbapenemase Genes of Carbapenem-Resistant Klebsiella pneumoniae Clinical Isolates from a Tertiary Care Teaching Hospital in Zunyi, China

, , , , , , & ORCID Icon show all
Pages 637-649 | Received 23 Nov 2022, Accepted 10 Jan 2023, Published online: 29 Jan 2023

Figures & data

Table 1 Basic Information on CRKP Isolates in Zunyi, China

Table 2 Antimicrobial Susceptibility of 94 CRKP Isolates

Table 3 The Occurrence of MDR, XDR and PDR Among the CRKP Isolates

Figure 1 Representative images of mCIM and eCIM test results. (A) mCIM test results; (B) eCIM test results. 1: ATCC®BAA-1705TM (producing serine carbapenemase) as a positive control; 2: CRKP clinical isolates (producing serine carbapenemase); 3: ATCC®BAA-2146TMa (producing metallo-β-lactamase) as a positive control; 4: CRKP clinical isolates (producing metallo-β-lactamase).

Abbreviations: mCIM, modified carbapenem inactivation method; eCIM, EDTA-carbapenem inactivation method.
Figure 1 Representative images of mCIM and eCIM test results. (A) mCIM test results; (B) eCIM test results. 1: ATCC®BAA-1705TM (producing serine carbapenemase) as a positive control; 2: CRKP clinical isolates (producing serine carbapenemase); 3: ATCC®BAA-2146TMa (producing metallo-β-lactamase) as a positive control; 4: CRKP clinical isolates (producing metallo-β-lactamase).

Figure 2 Prevalence of carbapenemase genes in CRKP isolates and the consistency between genotypes and phenotypes of the isolates.

Abbreviations: NDM, New Delhi metallo-β-lactamase; KPC, K. pneumoniae carbapenemase; VIM, Verona integron-encoded metallo-β-lactamase.
Figure 2 Prevalence of carbapenemase genes in CRKP isolates and the consistency between genotypes and phenotypes of the isolates.

Figure 3 Prevalence of virulence genes in the CRKP isolates.

Figure 3 Prevalence of virulence genes in the CRKP isolates.

Figure 4 Prevalence of carbapenemase genes in isolates from children and adults.

Abbreviations: NDM, New Delhi metallo-β-lactamase; KPC, K. pneumoniae carbapenemase; VIM, Verona integron-encoded metallo-β-lactamase. ***P <0.001.
Figure 4 Prevalence of carbapenemase genes in isolates from children and adults.

Figure 5 Sequence types of the CRKP isolates.

Abbreviation: ST, sequence type.
Figure 5 Sequence types of the CRKP isolates.

Figure 6 The proportion of carbapenemase genes in the top three CRKP STs.

Abbreviations: NDM, New Delhi metallo-β-lactamase; KPC, K. pneumoniae carbapenemase; VIM, Verona integron-encoded metallo-β-lactamase; ST, sequence type.
Figure 6 The proportion of carbapenemase genes in the top three CRKP STs.

Figure 7 Antimicrobial susceptibility of the top three STs. *P <0.05, ***P <0.001.

Abbreviation: ST, sequence type.
Figure 7 Antimicrobial susceptibility of the top three STs. *P <0.05, ***P <0.001.