Molecular Epidemiology and Characterization of Multidrug-Resistant MRSA ST398 and ST239 in Himachal Pradesh, India

Figures & data

Table 1 Primers Used in the Present Study for the Detection of Different Genes

No	Name of Gene	Primers	Tm ^°C	Amplicon Size (bp)	References
1	mecA	F:5′-GTAGAAATGACTGAACGTCCGATAA-3′	55	300	[22]
		R:5′-CCAATTCCACATTGTTTCGGTCTAA-3′
2	VanA	F:5′- ATCGTTGACATACATCGTTGCG-3′	54	421	[7]
		R:5′-GTA CAA TGT GGC CGTTA-3′
3	lukS-PV/ lukf-PV	F:5′-GAGACTATTTTGTGCCAGAC-3′	58	445	[22]
		R:5′-CACCTGACAAGCCGTTA-3′
4	16S rRNA	F:5′-GTAGGTGGCAAG CGTTATCC-3′	59	1008	[20]
		R:5′CGCACATCGCGTCAG-3′

Figure 1 Phylogenic analysis between antimicrobial susceptibility, molecular sequences typing and resistance determinant.

Note: White box indicates negative results.

Abbreviations: ST, Sequence type; CC, clonal complex; OXA, oxacillin; FOX, cefoxitin; ERY, erythromycin; CLI, clindamycin; TET, tetracycline; GEN, gentamicin; FA, fusidic acid; MOP, mupirocin; LNZ, linezolid; TEC, teicoplanin; CIP, ciprofloxacin; LNZ, levofloxacin; DAP, daptomycin; SXT, trimethoprim/sulfamethoxazole; QD, quinupristin-dalfopristin; DAL, dalbavancin; CPT, ceftaroline.

Figure 2 MRSA host specific, specimen and associated infection classification with PVL production.

Abbreviations: F, Female; M, Male; HA.MRSA, Hospital associated methicillin resistant Staphylococcus aureus; CA.MRSA, Community associated methicillin resistant Staphylococcus aureus; PVL, Panton–Valentine leukocidin.

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