The First Report of Escherichia coli and Klebsiella pneumoniae Strains That Produce Both NDM-5 and OXA-181 in Jiangsu Province, China

Figures & data

Table 1 The Minimum Inhibitory Concentration of Donor Bacteria, Recipient Bacteria and Conjugant (MIC, μg /Ml)

Antibiotics	EC73	J73	KP92	J92	KP100	J100	EC600
Ampicillin	≥32	≥32	≥32	≥32	≥32	≥32	4
Ampicillin/Sulbactam	≥32	≥32	≥32	≥32	≥32	≥32	≤2
Piperacillin	≥128	≥128	≥128	≥128	≥128	≥128	≤4
Piperacillin/tazobactam	≥128	≥128	≥128	64	≥128	64	≤4
Cefuroxime	≥64	≥64	≥64	≥64	≥64	≥64	4
Cefuroxime Axetil	≥64	≥64	≥64	≥64	≥64	≥64	4
Cefotetan	≥64	≥64	≥64	≥64	≥64	≥64	≤4
Ceftazidime	≥64	≥64	≥64	≥64	≥64	≥64	≤1
Ceftriaxone	≥64	≥64	≥64	≥64	≥64	≥64	≤1
Cefepime	≥64	16	≥32	32	≥32	32	≤1
Aztreonam	16	≤1	≤1	≤1	≤1	≤1	≤1
Imipenem	8	4	≥16	8	≥16	≥16	≤1
Meropenem	8	8	8	4	8	8	≤0.25
Amikacin	8	≤2	≤2	≤2	≤2	≤2	≤2
Gentamicin	≥16	≤1	≤1	≤1	≤1	≤1	≤1
Tobramycin	≥16	≤1	≤1	≤1	≤1	≤1	≤1
Ciprofloxacin	≥4	0.5	1	1	1	1	≤0.25
Levofloxacin	≥8	1	1	1	1	1	≤0.25
Trimethoprim/Sulfamethoxazole	≥320	≤20	≥320	≤20	≥320	≤20	≤20
Cefazolin	≥64	≥64	≥64	≥64	≥64	≥64	≤4
Nitrofurantoin	64	≤16	128	≤16	64	≤16	≤16
Colistin	≤0.5	≤0.5	≤0.5	≤0.5	≤0.5	≤0.5	≤0.5
Tigecycline	≤0.5	≤0.5	≤0.5	≤0.5	≤0.5	≤0.5	≤0.5

Notes: 1) In order to avoid duplication, only the first isolate was taken from the same specimen of the same child. In this study, a total of 3 strains were collected after removing the duplicate specimen: Escherichia coli (from sputum culture specimens) is denoted by EC73, Klebsiella pneumoniae (from blood culture specimens) is denoted by KP92, and Klebsiella pneumoniae (from ascites culture specimens) is denoted by KP100. Their conjugation products were denoted by J73, J92 and J100 respectively, and the donor bacteria was EC600. 2) The results for drug resistance have been bolded.

Figure 1 S1-PFGE profiles and Southern blotting results. From left to right: S1-PFGE profiles; Southern blotting results: The red arrow indicates the hybridization and localization of the plasmid carrying the bla_OXA-181 gene. Southern blotting results: The blue arrow indicates the hybridization and localization of the plasmid carrying the bla_NDM-5 gene.

Note: M: Salmonella H9812 Marker.

Figure 2 Comparison of NZ_CP024825.1, NZ_CP026727.1, KP100, KP92 and EC73 genomes from the outside in.

Figure 3 Comparative gene cluster analysis. Arrows indicate open reading frames arranged in the direction of transcription. The gene functions are represented by different colors. The red region represents drug resistance genes, the green region represents genes related to plasmid conjugation, the Orange regions represent insertion elements, the blue regions represent transposons, and the black regions encode other genes. Gray shaded areas indicate sequences that are highly similar sequences between plasmids.