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Infection and Drug Resistance Volume 16, 2023 - Issue
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ORIGINAL RESEARCH

Comparative Genomic Analysis of Hypervirulence Carbapenem-Resistant Klebsiella pneumoniae from Inpatients with Infection and Gut Colonization, China

Wan He1 School of Laboratory Medicine, Chengdu Medical College, Chengdu, 610500, People’s Republic of ChinaORCID Iconhttps://orcid.org/0000-0002-4619-5013
ORCID Icon,
Changbu Wu2 Department of Immunology and Microbiology, Advanced Medical Technology Center, The First Affiliated Hospital, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, 510080, People’s Republic of China;3 Key Laboratory of Tropical Diseases Control (Sun Yat-sen University), Ministry of Education, Guangzhou, 510080, People’s Republic of ChinaORCID Iconhttps://orcid.org/0009-0006-0005-4369
ORCID Icon,
Guanping Chen2 Department of Immunology and Microbiology, Advanced Medical Technology Center, The First Affiliated Hospital, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, 510080, People’s Republic of China;3 Key Laboratory of Tropical Diseases Control (Sun Yat-sen University), Ministry of Education, Guangzhou, 510080, People’s Republic of China;4 Department of Immunology, School of Medicine, Sun Yat-Sen University, Shenzhen, 518107, People’s Republic of China
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Guili Zhang2 Department of Immunology and Microbiology, Advanced Medical Technology Center, The First Affiliated Hospital, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, 510080, People’s Republic of China;3 Key Laboratory of Tropical Diseases Control (Sun Yat-sen University), Ministry of Education, Guangzhou, 510080, People’s Republic of China
,
Zihan Zhao2 Department of Immunology and Microbiology, Advanced Medical Technology Center, The First Affiliated Hospital, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, 510080, People’s Republic of China;3 Key Laboratory of Tropical Diseases Control (Sun Yat-sen University), Ministry of Education, Guangzhou, 510080, People’s Republic of China
,
Shu’an Wen2 Department of Immunology and Microbiology, Advanced Medical Technology Center, The First Affiliated Hospital, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, 510080, People’s Republic of China;3 Key Laboratory of Tropical Diseases Control (Sun Yat-sen University), Ministry of Education, Guangzhou, 510080, People’s Republic of China
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Yuan Zhou2 Department of Immunology and Microbiology, Advanced Medical Technology Center, The First Affiliated Hospital, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, 510080, People’s Republic of China;3 Key Laboratory of Tropical Diseases Control (Sun Yat-sen University), Ministry of Education, Guangzhou, 510080, People’s Republic of China
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Xue Deng2 Department of Immunology and Microbiology, Advanced Medical Technology Center, The First Affiliated Hospital, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, 510080, People’s Republic of China;3 Key Laboratory of Tropical Diseases Control (Sun Yat-sen University), Ministry of Education, Guangzhou, 510080, People’s Republic of China
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Yu Feng2 Department of Immunology and Microbiology, Advanced Medical Technology Center, The First Affiliated Hospital, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, 510080, People’s Republic of China;3 Key Laboratory of Tropical Diseases Control (Sun Yat-sen University), Ministry of Education, Guangzhou, 510080, People’s Republic of China
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Lan-Lan Zhong2 Department of Immunology and Microbiology, Advanced Medical Technology Center, The First Affiliated Hospital, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, 510080, People’s Republic of China;3 Key Laboratory of Tropical Diseases Control (Sun Yat-sen University), Ministry of Education, Guangzhou, 510080, People’s Republic of ChinaORCID Iconhttps://orcid.org/0009-0005-3311-5829
ORCID Icon,
Guo-Bao Tian2 Department of Immunology and Microbiology, Advanced Medical Technology Center, The First Affiliated Hospital, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, 510080, People’s Republic of China;3 Key Laboratory of Tropical Diseases Control (Sun Yat-sen University), Ministry of Education, Guangzhou, 510080, People’s Republic of China;4 Department of Immunology, School of Medicine, Sun Yat-Sen University, Shenzhen, 518107, People’s Republic of ChinaCorrespondence[email protected]
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Min Dai1 School of Laboratory Medicine, Chengdu Medical College, Chengdu, 610500, People’s Republic of ChinaCorrespondence[email protected]
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Pages 5251-5261 | Received 25 May 2023, Accepted 12 Jul 2023, Published online: 14 Aug 2023

Figures & data

Figure 1 Genomic characteristics of 46 gut colonized hv-CRKP strains. Characteristics of virulence genes and drug resistance genes in 46 gut colonized hv-CRKP strains.

Figure 1 Genomic characteristics of 46 gut colonized hv-CRKP strains. Characteristics of virulence genes and drug resistance genes in 46 gut colonized hv-CRKP strains.

Figure 2 The virulence characteristics of 46 gut colonized hv-CRKP strains. (A) Virulence of 46 gut colonized hv-CRKP strains to BALB/C mice. (B) Viscosity characteristics of 46 gut colonized hv-CRKP strains. *P<0.05; **P<0.01; ***P<0.001; ****P<0.0001.

Abbreviation: ns, No statistical significance.
Figure 2 The virulence characteristics of 46 gut colonized hv-CRKP strains. (A) Virulence of 46 gut colonized hv-CRKP strains to BALB/C mice. (B) Viscosity characteristics of 46 gut colonized hv-CRKP strains. *P<0.05; **P<0.01; ***P<0.001; ****P<0.0001.

Figure 3 Phylogenetic tree of gut colonized hv-CRKP and pathogenic hv-CRKP. (A and B) Phylogenetic analysis of gut colonized ST11 hv-CRKP and pathogenic ST11 hv-CRKP based on core genome SNPs. SCs are distinguished by different colors. Clade1 (blue oval) was SC5, and Clade 2 (red oval) included SC1, SC2, SC3, and SC4.

Figure 3 Phylogenetic tree of gut colonized hv-CRKP and pathogenic hv-CRKP. (A and B) Phylogenetic analysis of gut colonized ST11 hv-CRKP and pathogenic ST11 hv-CRKP based on core genome SNPs. SCs are distinguished by different colors. Clade1 (blue oval) was SC5, and Clade 2 (red oval) included SC1, SC2, SC3, and SC4.

Figure 4 Genomic characteristics of gut colonized hv-CRKP and pathogenic hv-CRKP. (A) Virulence genes and carbapenem resistance genes carried by ST11 hv-CRKP among different SCs. (B) COG classification annotation results of accessory genome differential genes of gut colonized ST11 hv-CRKP and pathogenic ST11 hv-CRKP.

Figure 4 Genomic characteristics of gut colonized hv-CRKP and pathogenic hv-CRKP. (A) Virulence genes and carbapenem resistance genes carried by ST11 hv-CRKP among different SCs. (B) COG classification annotation results of accessory genome differential genes of gut colonized ST11 hv-CRKP and pathogenic ST11 hv-CRKP.

Figure 5 Gene map of virulence plasmids harbored by gut colonized hv-CRKP strains. (A) The structural comparison between virulence plasmid pKP01_vir, pKP41_vir, pKP20194a-p1 and pKP2044 (B) The genetic environment of the pKPC2_KP01 resistance gene blaKPC-2 and the pNDM_KP41 resistance gene blaNDM-1.

Figure 5 Gene map of virulence plasmids harbored by gut colonized hv-CRKP strains. (A) The structural comparison between virulence plasmid pKP01_vir, pKP41_vir, pKP20194a-p1 and pKP2044 (B) The genetic environment of the pKPC2_KP01 resistance gene blaKPC-2 and the pNDM_KP41 resistance gene blaNDM-1.

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