Development and Validation of Multiplex Quantitative PCR Assay for Detection of Helicobacter pylori and Mutations Conferring Resistance to Clarithromycin and Levofloxacin in Gastric Biopsy [Corrigendum]

Figures & data

Table 1 Oligonucleotides Sequence Used in This Study

Primers/Probes	Sequences (5′→3′)	Targets	Product (bp)
UreA-F^a	AGTTCCTGGTGAGTTGTTCTT	ureA	120
UreA-R^b	TGGAAGTGTGAGCCGATTT
Cla-F^a	CTGCGCATGATATTCCCGTT	23S rDNA	157
Cla-R^b	CGACCTGCATGAATGGCGTA
Lev-F^a	CGCCATCAATAGAGCCAAAG	gyrA	136
Lev-R^b	GTGGGTGATGTGATTGGTAAAT
UreA-P^c	FAM-AAATGTTGGCGACAGACCGGTTC-TAMRA	ureA
Cla-PWT^c	Cy5-AAGGTCCACGGGGTCTTTCCGTC-BHQ2	Wild type
Cla-P2142G^c	HEX-AGGTCCACGGGGTCTTCCCGTCT-BHQ2	A2142G
Cla-P2143G^c	FAM-AGGTCCACGGGGTCTCTCCGTCTT-BHQ2	A2143G
Lev-N87WT^c	HEX-CCG+C+G+TTA+T+CG-IowaBlack^®FQ	Wild type
Lev-N87K^c	6-FAM-CCG+C+T+TT+A+T+CGC-IowaBlack^®FQ	N87K
Lev-N87I^c	Cy5-CCG+C+A+AT+A+T+CGC-IowaBlack^®RQ-Sp	N87I
Lev-D91WT^c	HEX-TA+T+G+AT+GCG+CT-IowaBlack^®FQ	Wild type
Lev-D91N^c	Cy5-TTA+T+A+ATG+C+G+CTAGT-IowaBlack^®RQ-Sp	D91N
Lev-D91Y^c	6-FAM-TTA+T+T+ATG+C+G+CTAGT-IowaBlack^®FQ	D91Y

Note: ^aForward primer, ^bReverse primer, ^cTaqMan probe.