Molecular Epidemiological Characteristics of Carbapenem Resistant Aeromonas from Hospital Wastewater

Figures & data

Table 1 Antimicrobial Susceptibility Analysis of Aeromonas caviae and Aeromonas veronii

Drugs	Aeromonas caviae (n=16)			Aeromonas veronii (n=17)			p-value
	Susceptible	Intermediate	Resistant	Susceptible	Intermediate	Resistant
TZP	-	62.5%	37.5%	5.9%	58.8%	35.3%	>0.05
FOX	12.5%	-	87.5%	5.9%	29.4%	64.7%	>0.05
CRO	-	-	100.0%	-	-	100.0%	-
FEP	-	-	100.0%	5.9%	-	94.1%	>0.05
ATM	-	-	100.0%	11.8%	-	88.2%	>0.05
ETP	-	-	100.0%	-	-	100.0%	-
IPM	-	-	100.0%	5.9%	-	94.1%	>0.05
AN	68.7%	6.3%	25.0%	41.2%	23.5%	35.3%	>0.05
CN	56.2%	-	43.8%	23.5%	17.6%	58.8%	>0.05
CIP	12.5%	31.3%	56.3%	47.1%	23.5%	29.4%	>0.05
LVX	56.2%	31.3%	12.5%	88.2%	11.8%	-	>0.05
SXT	43.7%	-	56.3%	64.7%	-	35.3%	>0.05

Table 2 The Frequency of Carbapenemase Genes Among Aeromonas caviae and Aeromonas veronii

Genes	Total population; n(%)	Aeromonas caviae; n(%)	Aeromonas veronii n(%)	p
blaKPC	32 (97.0)	16 (100.0)	16 (94.1)	>0.05
cphA	13 (39.4)	–	13 (76.5)	<0.05

Figure 1 ERIC-PCR analysis of Aeromonas caviae (a) and Aeromonas veronii (b).

Figure 2 Phylogenetic tree analysis of Aeromonas caviae (a) and Aeromonas veronii (b).

Figure 3 Results of plasmid stability for bla_KPC-IncP-6 and bla_KPC-IncFII in E.coli DH5α.

Table 3 Different Types of bla_KPC-Carrying Plasmids and Their Fitness Cost Results

Plasmid	Inc group	Host	Relative Adaptive Fitness
p3a64	IncP-6	E.coli DH5α	1.024
p15a82	IncP-6	E.coli DH5α	1.018
p25a85	IncP-6	E.coli DH5α	0.981
p12	IncFII	E.coli DH5α	0.962
p31	IncFII	E.coli DH5α	1.016
p1008	IncFII	E.coli DH5α	1.039

Figure 4 bla_KPC regions from IncP-6 plasmids and comparison with the related plasmids.

Data Sharing Statement

The raw data supporting the conclusions of this article will be made available by the authors, without undue reservation.