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ORIGINAL RESEARCH

The Combination of 3-Hydrazinoquinoxaline-2-Thiol with Thymoquinone Demonstrates Synergistic Activity Against Different Candida Strains

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Pages 2289-2298 | Received 23 Mar 2024, Accepted 22 May 2024, Published online: 06 Jun 2024

Figures & data

Table 1 MICs of Thymoquinone and 3-Hydrazinoquinoxaline-2-Thiol in μg/mL for Variety of Clinical Candida Species

Figure 1 (A) Minimum inhibitory concentration (MIC) of 3-hydrazinoquinoxaline-2-thiol was 32 µg/mL and it was reduced to 8 µg/mL (4 fold) when it was combined with thymoquinone against C. albicans 2 (B) MIC of thymoquinone was 16 µg/mL and it was reduced to 1 µg/mL (16 fold) when it was combined with 3-hydrazinoquinoxaline-2-thiol against Candida 2. T represents thymoquinone and Q represents 3-hydrazinoquinoxaline-2-thiol. The experiment was performed in triplicate, and the average value was calculated.

Figure 1 (A) Minimum inhibitory concentration (MIC) of 3-hydrazinoquinoxaline-2-thiol was 32 µg/mL and it was reduced to 8 µg/mL (4 fold) when it was combined with thymoquinone against C. albicans 2 (B) MIC of thymoquinone was 16 µg/mL and it was reduced to 1 µg/mL (16 fold) when it was combined with 3-hydrazinoquinoxaline-2-thiol against Candida 2. T represents thymoquinone and Q represents 3-hydrazinoquinoxaline-2-thiol. The experiment was performed in triplicate, and the average value was calculated.

Table 2 The Interaction of 3-Hydrazinoquinoxaline-2-Thiol with Thymoquinone Against Diverse Candida Strains in vitro. Synergy is Considered When the Fractional Inhibitory Concentration Index (FICI) Falls Below 0.5

Figure 2 Checkerboard assay demonstrating the synergistic effect of 3-hydrazinoquinoxaline-2-thiol with thymoquinone against Candida albicans 3. The dark blue color represents 100% growth and the white color represent 0% growth.

Figure 2 Checkerboard assay demonstrating the synergistic effect of 3-hydrazinoquinoxaline-2-thiol with thymoquinone against Candida albicans 3. The dark blue color represents 100% growth and the white color represent 0% growth.