Figures & data
Figure 1 Three-dimensional structures of human antimicrobial peptides.
Notes: The Protein Data Bank identification for these structures are 3GNY for dimeric crystal structure of human α-defensin 1 (or human neutrophil peptide-1); 1E4S for human beta defensin 1; and 2K6O for human cathelicidin LL-37 in complex with sodium dodecyl sulfate micelles. Structural coordinates were obtained from the Research Collaboratory for Structural Bioinformatics Protein Databank (http://www.rcsb.org). The significance of ‘1’ and ‘2’is for dimeric crystal structure of human α-defensin 1 (two peptides: ‘1’ and ‘2’, together).
Figure 2 Representative structures of α-AA and γ-AApeptides.
Figure 3 General building block strategy for the synthesis of AApeptides.
Notes: Each coupling cycle includes an Fmoc deprotection using 20% piperidine in DMF and coupling of α-AA or γ-AApeptides building blocks onto resin in the presence of DIC/ODhbt in DMF. After desired sequences are assembled, they are cleaved.
Abbreviations: DIC, diisopropylcarbodiimide; DMF, dimethyl fluoride; Fmoc, 9- fluorenylmethyloxycarbonyl; ODhbt, 3-hydroxy-4-oxo-3,4-dihydro-1,2,3-benzotriazine; γ-AA, γ-AApeptides; α-AA, α-AApeptides.
