Genetic relatedness and host specificity of Pseudomonas aeruginosa isolates from cystic fibrosis and non-cystic fibrosis patients

Figures & data

Table 1 Number of P. aeruginosa isolates and their respective clusters on PFGE typing

Patient number	Source of P. aeruginosa	Number of P. aeruginosa isolates per patient	Cluster 1: CF (n=16) and environment (n=2)	Cluster 2: CF (n=11), non-CF (n=1), and environment (n=1)	Cluster 3: CF (n=2), non-CF (n=12), and environment (n=1)	Cluster 4: CF (n=3) and environment (n=1)
1	CF	4	2	2
2	CF	1	1
3	CF	1	1
4	CF	1	1
5	CF	2	1	1
6	CF	2	1	1
7	CF	2	2
8	CF	2	2
9	CF	1	1
10	CF	1	1
11	CF	1	1
12	CF	1	1
13	CF	1	1
14	CF	1		1
15	CF	1		1
16	CF	3		1	2
17	CF	1		1
18	CF	1		1
19	CF	2		1		1
20	CF	2		1		1
21	CF	1				1
22	Non-CF	1		1
23	Non-CF	1			1
24	Non-CF	1			1
25	Non-CF	1			1
26	Non-CF	1			1
27	Non-CF	1			1
28	Non-CF	1			1
29	Non-CF	1			1
30	Non-CF	1			1
31	Non-CF	1			1
32	Non-CF	1			1
33	Non-CF	1			1
34	Non-CF	1			1
Environmental isolate number
1	Environment	1	1
2	Environment	1	1
3	Environment	1		1
4	Environment	1			1
5	Environment	1				1

Abbreviations: P. aeruginosa, Pseudomonas aeruginosa; PFGE, pulsed-field gel electrophoresis; CF, cystic fibrosis.

Figure 1 Dendrogram based on percentage similarities among P. aeruginosa isolates obtained using PFGE fingerprinting.

Abbreviations: P. aeruginosa, Pseudomonas aeruginosa; PFGE, pulsed-field gel electrophoresis; XbaI, restriction enzyme isolated from Xanthomonas badrii.

Table 2 Number of P. aeruginosa isolates resistant to common antimicrobial agents in vitro and their respective clusters after PFGE typing

Antibiotics	Distribution of resistant P. aeruginosa – PFGE clusters*
	Cluster 1: CF resistance (n=6/16)	Cluster 2: CF resistance (n=5/11)	Cluster 3: CF resistance (n=2/2) and non-CF resistance (n=8/12)	Cluster 4: CF resistance (n=3/3)
Ciprofloxacin	3	4	6	2
Amikacin	2	4	4	2
Gentamicin	2	4	4	2
Cefepime	1	1	2	1
Ceftazidime	0	0	2	1
Meropenem/imipenem	0	0	8 (non-CF)	0
Piperacillin/tazobactam	0	0	1	0
Colistin	1	0	0	0

Notes:

* Some isolates resistant to ≥2 antibiotics.

Abbreviations: P. aeruginosa, Pseudomonas aeruginosa; PFGE, pulsed-field gel electrophoresis; CF, cystic fibrosis.