Discrepancy in MALDI-TOF MS identification of uncommon Gram-negative bacteria from lower respiratory secretions in patients with cystic fibrosis

Figures & data

Table 1 Discrepancy in the identification of bacterial isolates by different methods

Phenotypic identification (strain number)	MALDI-TOF MS identification	16S rRNA gene sequence	GenBank accession number
Haemophilus parainfluenzae (1612506366)	Haemophilus parahaemolyticus	Haemophilus paraphrohaemolyticus	KJ128299
Haemophilus species (1612505787)	Haemophilus parahaemolyticus	Haemophilus paraphrohaemolyticus	KJ128300
Achromobacter species I (1612508591)	Achromobacter xylosoxidans	Achromobacter xylosoxidans	KJ128296
Achromobacter species II (1612509043)	Achromobacter xylosoxidans	Achromobacter xylosoxidans	KJ128298
Ralstonia pickettii (1612507054)	Achromobacter xylosoxidans	Achromobacter insolitus	KJ128297

Abbreviations: MALDI-TOF MS, matrix-assisted laser desorption/ionization-time of flight mass spectrometry; rRNA, ribosomal RNA.

Figure 1 Identification of bacterial species by conventional phenotypic and MALDI-TOF MS methods.

Notes: The organisms listed are Staphylococcus aureus, Pseudomonas aeruginosa, Haemophilus influenzae, Streptococcus pyogenes, Achromobacter xylosoxidans, Moraxella catarrhalis, Haemophilus parainfluenzae, Enterobacter cloacae, Chryseobacterium gleum, Streptococcus pneumoniae, Stenotrophomonas maltophilia, Ralstonia pickettii, and Haemophilus parahaemolyticus.

Abbreviation: MALDI-TOF MS, matrix-assisted laser desorption/ionization-time of flight mass spectrometry.