https://orcid.org/0000-0003-1313-6217
Figures & data
Figure 1 CircRNA_104565 was highly expressed in PTC tissue and cell. (A) GEO dataset (GSE93522) showed that circRNA_104565 was significantly increased in PTC tissues compared to normal tissues. (B) CircRNA_104565 expression was significantly increased in PTC tissues compared to normal tissues. (C) CircRNA_104565 was significantly increased in PTC cells compared to Nthy-ori-3–1. *P < 0.05.
Figure 2 Silencing circRNA_104565 inhibited cell proliferation in PTC cell. (A) CircRNA_104565 expressions were detected by qRT-PCR. (B) Cell proliferations were detected by CCK8 assay. (C) The tumor volumes were measured every 5 days. (D) Tumor weight was measured after mouse was surgically dissected. *P < 0.05.
Figure 3 CircRNA_104565 acts as a ceRNA for miR-134 to promote cell proliferation in PTC. (A) The potential binding sites of circRNA_104565 and miR-134 were predicted by miRDB and starbase tool. (B) Luciferase reporter assay had confirmed complementary combinations between circRNA_104565 and miR-134. (C) MiR-134 expressions were detected by qRT-PCR. (D) MiR-134 expression was significantly decreased in PTC tissues compared to normal tissues. (E) Pearson correlation analysis revealed circRNA_104565 expression was inversely correlated with miR-134 in PTC tissue. (F) Cell proliferations were detected by CCK8 assay. *P < 0.05.
Figure 4 CircRNA_104565 accelerates PTC cell growth by regulating miR-134/ELF2 axis. (A) MiR-134 had potential interaction with ELF2 via a biological website. (B) Luciferase reporter assay had confirmed complementary combinations between ELF2 and miR-134. (C) ELF2 expression was significantly increased in PTC tissues compared to normal tissues. (D) Pearson correlation analysis revealed ELF2 expression was inversely correlated with miR-134. (E) Pearson correlation analysis revealed ELF2 expression was positively correlated with circRNA_104565. ELF2 expression were detected by qRT-PCR (F) and Western blot (G) (H) Cell proliferations were detected by CCK8 assay. *P < 0.05.
