The Lymphocyte Depletion in Patients with Acute Ischemic Stroke Associated with Poor Neurologic Outcome

Figures & data

Table 1 Demographic Characteristics, Risk Factor, Leukocyte Count, Differential Leukocyte Count, Infarct Location, and NIHSS

Variables	N = 193
Gender
Male	98 (50.8%)
Female	95 (49.2%)
Age
< 60 years	126 (65.3%)
≥ 60 years	67 (34.7%)
Risk factor
1 Risk factor	18 (9.3%)
≥2 risk factors	175 (90.7%)
Leukocyte (cells/uL)
Mean ± Std	9337.01±2835.224
Median	9110.00
Range (min-max)	516.00–19,140.00
Basophil (%)
Mean ± Std	0.00
Median	0.00
Range (min-max)	0.00
Eosinophil (%)
Mean ± Std	1.60±2.096
Median	1.00
Range (min-max)	0.00–13.00
Banded neutrophil (%)
Mean ± Std	0.04±0.311
Median	0.00
Range (min-max)	0.00–3.00
Segmented neutrophil (%)
Mean ± Std	69.93±12.652
Median	70.00
Range (min-max)	7.00–94.00
Lymphocyte (%)
Mean ± Std	20.87±9.944
Median	20.00
Range (min-max)	4.00–52.00
Monocyte (%)
Mean ± Std	7.25±3.281
Median	7.00
Range (min-max)	1.00–26.00
Neutrophil Lymphocyte Ratio
Mean ± Std	4.81±3.912
Median	3.45
Range (min-max)	0.50–23.50
Infarct location
Subcortical	156 (80.8%)
Cortical	22 (11,4%)
Pons	15 (7.8%)
NIHSS at admission
Mean±Std	6.49±3.200
Median	6.00
Range (min-max)	1.00–19.00
NIHSS degree at admission
Mild	66 (34.2%)
Moderate	121 (62.7%)
Severe	6 (3.1%)
NIHSS at discharge
Mean±Std	5.44±2.925
Median	5.00
Range (min-max)	0.00–16.00
NIHSS degree at discharge
Mild	83 (43.0%)
Moderate	109 (56.5%)
Severe	1 (0.5%)

Table 2 The Comparison of Differential Counts by Risk Factor

Variables	Risk Factor		P value
	1 Risk Factor (N=18)	≥ 2 Risk Factors (N=175)
Leukocyte (cells/uL)			0.276
Mean ± Std	9893.33±2779.528	9279.79±2842.523
Median	9520.00	9080.00
Range (min-max)	4880.00–16,830.00	516.00–19,140.00
Basophil (%)			1.00
Mean ± Std	0.00	0.00
Median	0.00	0.00
Range (min-max)	0.00	0.00
Eosinophil (%)			0.929
Mean ± Std	1.33±1.236	1.63±2.166
Median	1.00	1.00
Range (min-max)	0.00–4.00	0.00–13.00
Banded neutrophil (%)			0.389
Mean±Std	0.16±0.707	0.03±0.237
Median	0.00	0.00
Range (min-max)	0.00–3.00	0.00–2.00
Segmented neutrophil (%)			0.788
Mean±Std	69.16±11.089	70.01±12.828
Median	67.50	71.00
Range (min-max)	45.00–94.00	7.00–93.00
Lymphocyte (%)			0.821
Mean±Std	19.88±8.137	20.97±10.126
Median	20.50	20.00
Range (min-max)	4.00–37.00	4.00–52.00
Monocyte (%)			0.636
Mean±Std	8.94±6.053	7.08±2.824
Median	6.50	7.00
Range (min-max)	2.00–26.00	1.00–25.00

Table 3 The Comparison of Differential Counts by Leukocyte Level

Variables	Leukocyte Category		P value
	> 10,000 (N=66)	≤10,000 (N=127)
Leukocyte (cells/uL)			0.000**
Mean±Std	12,341.06±2093.478	7775.85±1674.718
Median	11,570.00	7970.00
Range (min-max)	10,010.00–19,140.00	516.00–10,000.00
Basophil (%)			1.00
Mean±Std	0.00	0.00
Median	0.00	0.00
Range (min-max)	0.00	0.00
Eosinophil (%)			0.071
Mean±Std	1.19±1.490	1.81±2.327
Median	1.00	1.00
Range (min-max)	0.00–6.00	0.00–13.00
Banded neutrophil (%)			0.778
Mean±Std	0.06±0.387	0.03±0.264
Median	0.00	0.00
Range (min-max)	0.00–3.00	0.00–2.00
Segmented neutrophil (%)			0.000**
Mean±Std	73.81±13.405	67.91±11.799
Median	73.50	69.00
Range (min-max)	7.00–94.00	29.00–93.00
Lymphocyte (%)			0.001**
Mean±Std	17.71±8.961	22.51±10.064
Median	17.50	23.00
Range (min-max)	4.00–41.00	4.00–52.00
Monocyte (%)			0.855
Mean±Std	7.43±3.791	7.16±2.994
Median	7.00	7.00
Range (min-max)	1.00–26.00	2.00–25.00

Note: **Mann–Whitney test significance at p<0.001.

Table 4 Comparison Between Differential Counts by Location of Infarction

Variables		Infarct Location		P value
	Subcortical (N=156)	Cortical (N=22)	Pons (N=15)
Leukocyte (cells/uL)				0.877
Mean±Std	9287.93±2463.710	9812.27±4109.448	9150.40±4159.565
Median	9095.00	9705.00	9000.00
Range (min-max)	3230.00–18,120.00	2540.00–19,140.00	516.00–18,980.00
Basophil (%)				1.00
Mean±Std	0.00	0.00	0.00
Median	0.00	0.00	0.00
Range (min-max)	0.00	0.00	0.00
Eosinophil (%)				0.925
Mean±Std	1.67±2.226	1.36±1.559	1.26±1.222
Median	1.00	1.00	1.00
Range (min-max)	0.00–13.00	0.00–5.00	0.00–4.00
Banded neutrophil (%)				0.546
Mean±Std	0.05±0.345	0.00	0.00
Median	0.00	0.00	0.00
Range (min-max)	0.00–3.00	0.00	0.00
Segmented neutrophil (%)				0.278
Mean±Std	69.35±12.028	70.72±17.989	74.73±8.883
Median	70.00	72.50	72.00
Range (min-max)	29.00–94.00	7.00–90.00	62.00–90.00
Lymphocyte (%)				0.356
Mean±Std	21.23±10.073	17.95±9.805	21.46±8.559
Median	20.00	16.00	20.00
Range (min-max)	4.00–52.00	5.00–36.00	10.00–41.00
Monocyte (%)				0.316
Mean±Std	7.16±3.287	7.90±3.084	7.26±3.594
Median	7.00	8.00	6.00
Range (min-max)	1.00–26.00	2.00–15.00	2.00–17.00

Table 5 Correlation Between NIHSS Degree Score at Admission, NIHSS Degree Score at Discharge, and Abnormal Differential Count

Variables	NIHSS Degree Score at Admission	NIHSSA Degree at Discharge
Leukocyte	R= 0.018, P value= 0.886	R=0.015 P value= 0.905
Basophil
Eosinophil	R= −0.165 P value= 0.572	R=−0.112 P value= 0.702
Banded neutrophil	R= 0.019 P value= 0.796	R= 0.05 P value= 0.49
Segmented neutrophil	R= −0.143 P value= 0.217	R= −0.104 P value= 0.371
Lymphocyte	R= 0.245 P value= 0.033*	R= 0.189 P value=0.102
Monocyte	R= 0.039 P value= 0.785	R= 0.003 P value=0.985

Notes: R: correlation coefficient, *Significance at p<0.05.