Prognostic Value of c-MYC Expression in Patients with Peripheral Neuroblastic Tumors

Figures & data

Table 1 MYCN Gene Expression Detected by FISH and c-MYC Protein Expression by IHC in NTs

c-MYC	MYCN			Total
	MNA	SAC	Non-MNA
(+)	0	1	15	16
(±)	0	0	0	0
(-)	28	4	129	161
Total	28	5	144	177

Abbreviations: MNA, MYCN amplification; SAC, suspicious amplification cases; non-MNA, cases without MYCN amplification.

Figure 1 MYCN gene expression detected by FISH and c-MYC protein expression by IHC in NTs. (A) MYCN amplification, FISH, original magnification × 1000. MYCN (red signal) clustering occurred. (B) Lack of MYCN amplification, FISH, original magnification × 1000. The ratio of MYCN (red signal) to LAF (green signal) was ≤1.0. (C) Positive (+) c-MYC expression, immunostaining, original magnification × 400. (D) Negative (-) c-MYC expression, immunostaining, original magnification × 400.

Table 2 Association of MYCN Amplification and c-MYC Expression with the Shimada Classification

	MYCN (Amplified)	MYCN (Not Amplified)	c-MYC (+)	c-MYC (-)
FH type	2	119	13	108
uFH type	26	30	3	53
P value^a	0.0001		0.3977
<1.5 year-old	1	53	4	50
≥1.5 year-old	27	96	12	111
P value^a	0.0001		0.7790

Notes: ^aFrom Fisher’s test.

Table 3 Association of MYCN Amplification and Positive c-MYC Expression with Morphological Changes

	Morphological Changes Related to Poor Prognosis		Morphological Changes Related to Good Prognosis
	Bull’s Eye Cells	Nuclear Inclusions	Calcification	Lymphocyte Clustering
MYCN (Amplified)	26/28	3/28	0	0
c-MYC (+)	0	0	0	1/16

Figure 2 Morphological changes of NTs. HE staining, original magnification × 400. (A) Bull’s eye cells (indicated by arrows). (B) Nuclear inclusions (indicated by arrows). (C) Calcification (indicated by arrows). (D) Lymphocyte clustering (indicated by arrows).

Table 4 Association of MYCN Amplification and c-MYC Expression with the 3-Year Survival Rate in Patients with NTs

	MNA	Non-MNA	c-MYC (+)	c-MYC (-)
FH type	46%	90%	43%	87%
uFH type	41%	53%	44%	55%

Abbreviations: MNA, MYCN amplification; non-MNA, cases without MYCN amplification.

Figure 3 Kaplan-Meier curves and Log rank tests. The results showed that there was a significant difference in survival rate between the c-myc positive and negative groups (P=0.001).