Correlation Between Vitamin D Status and HBsAg Antibody Levels in Indonesian Adolescents Immunised Against Hepatitis B

Figures & data

Table 1 Research Subjects’ Characteristics (n = 50)

Characteristic	Statistical Measure
1. Sex:
Male	23
Female	27
2. Age (years):
10–12	19
13–17	31
Average (SD)	12.9 (2.2)
Range	10–17
3. Weight (kg):
Average (SD)	44.2 (12.8)
4. Height (cm):
Average (SD)	150.5 (12.0)
5. Body mass index (kg/m^2):
<18.5	21
18.5–25.0	25
>25.0	4
Average (SD)	19.11 (3.44)

Table 2 Subjects’ 25(OH)D and Anti-HBsAg Levels After Complete Vaccination

Variable	Statistical Measure			Normality Test (p-value*)
	Average (SD)	Median	Range
1. 25(OH)D (ng/mL)	14.57 (5.99)	13.65	6.5–27.9	0.008
2. Anti-HBsAg levels before (mIU/mL)	15.68 (42.33)	5.50	0.00–247.74	<0.001
3. Anti-HBsAg levels after (mIU/mL)	9064.6 (11583.6)	3921.0	124.47–50940.25	<0.001

Note: *Shapiro–Wilk test.

Table 3 Subjects’ 25(OH)D Status and Pre- and Post-HBV Immunisation Antibody Responses

Variable	Number	%
25(OH)D status (ng/mL):
Endocrine
Deficiency (≤ 20)	40	80
Insufficiency (21.00–29.99)	10	20
Pre-immunisation HBV antibody response (mIU/mL):
<10	37	74
≥10	13	26
Post-immunisation HBV antibody response (mIU/mL):
<10	0	0
≥10	50	100

Table 4 Relationship with 25(OH)D Status

25(OH)D Status	Sex		p	Age (Years)		p
	Male	Female		10–13	14–17
Endocrine
Deficiency	17	23	0.001*	22	18	0.279
Insufficiency	10	0		8	2
Median	9.8	18.2	<0.001*	15.2	11.8	0.090
Range	6.5–19.6	6.7–27.9		6.5–27.9	6.5–23.3

Note: *The p-values were calculated using the Chi-square test except when comparing median vitamin D levels, which used the Mann–Whitney test.

Table 5 Relationship Between 25(OH)D Status and Anti-HBsAg Levels

25(OH)D Status	Pre-Immunisation Anti-HBsAg Levels		Post-Immunisation Anti-HBsAg Levels
	Median (Range)	p*	Median (Range)	p*
Endocrine
Deficiency	5.39 (0.00–247.74)	0.574	3852.0 (124.5–50940.2)	0.784
Insufficiency	8.06 (0.24–21.56)		4538.8 (152.7–46203.0)

Note: *Kruskal–Wallis/Mann–Whitney test.