Relationship Between MUC4 Variants and Metastatic Recurrence in Colorectal Cancer

Figures & data

Table 1 Baseline Characteristics of Case and Control Group (n,%)

Parameter	Total (n=84)	Case Group (n=47)	Control Group (n=37)	P
Gender				0.759
Female	37 (44.0%)	20 (42.6%)	17 (45.9%)
Male	47 (56.0%)	27 (57.4%)	20 (54.1%)
Age				0.922
<60years	30 (35.7%)	17 (36.2%)	13 (35.1%)
≥60years	54 (64.3%)	30 (63.8%)	24 (64.9%)
Tumour Localization				0.013
Colon	20 (23.8%)	16 (34.0%)	4 (10.8%)
Rectum	64 (76.2%)	31 (66.0%)	33 (89.2%)
T Stage				0.623
T1-T2	14 (16.7%)	7 (14.9%)	7 (18.9%)
T3-T4	70 (83.3%)	40 (85.1%)	30 (81.1%)
N Stage				<0.001
N0	32 (38.1%)	10 (21.3%)	22 (59.5%)
N1-N2	52 (61.9%)	37 (78.7%)	15 (40.5%)
Nodules				0.117
Yes	18 (21.4%)	13 (27.7%)	5 (13.5%)
No	66 (78.6%)	34 (72.3%)	32 (86.5%)
Perineural invasion				0.443
Yes	8 (9.5%)	6 (12.8%)	2 (5.4%)
No	76 (90.5%)	41 (87.2%)	35 (94.6%)
Lymphovascular invasion				0.039
Yes	12 (14.3%)	10 (21.3%)	2 (5.4%)
No	72 (85.7%)	37 (78.7%)	35 (94.6%)
MUC4 Mutations
rs56359992				0.019
Yes	37 (44.0%)	26 (55.3%)	11 (29.7%)
No	47 (56.0%)	21 (44.7%)	26 (70.3%)
rs1560322790				0.267
Yes	18 (21.4%)	8 (17.0%)	10 (27.0%)
No	66 (78.6%)	39 (83.0%)	27 (73.0%)
rs781124621				0.381
Yes	11 (13.1%)	8 (17.0%)	3 (8.1%)
No	73 (86.9%)	39 (83.0%)	34 (91.9%)

Figure 1 Correlation between MUC4 variants and prognosis in CRC patients.(A) Kaplan–Meier DFS curves for CRC patients with rs56359992 and without rs56359992. (B) Kaplan–Meier DFS curves for CRC patients with rs1560322790 and without rs1560322790. (C) Kaplan–Meier DFS curves for CRC patients with rs781124621 and without rs781124621.

Figure 2 Relationship between MUC4 variants and CRC metastasis after radical surgery. The barplot showed the proportion of CRC patients with metastasis. **p<0.01.

Figure 3 Relationship between variant rs781124621 and peritoneal metastasis. ****p<0.0001.

Figure 4 Analysis of biological activity associated with MUC4 mutations. (A) The volcano plot of the DEGs. (B) Gene ontology enrichment analysis. (C) Kyoto Encyclopedia of Genes and Genome pathway enrichment analysis. (D) Gene set enrichment analysis.

Figure 5 Immunescore of MUC4 mutated group and MUC4 WT group. *p<0.05.

Figure 6 Distributions of immune cells in MUC4 mutated group and MUC4 WT group. *p<0.05; **p<0.01.