A20 Promoter rs5029924 Concomitant with rs2230926 and rs5029937 May Be a Prognostic Predictor for Joint Deformity or Refractory Rheumatoid Arthritis

Figures & data

Figure 1 A20 rs5029924, rs5029937 and rs2230926 identified in RA patients. (A) Experimental procedure in this study; (B) rs5029924, rs5029937 and rs2230926 identification in a RA patient. The arrows indicate the site of nucleotide change. Wild: Wild-type; Heter: Heterozygous.

Table 1 Frequencies of the rs5029924, rs2230926, and rs5029937 Alleles and Genotypes in RA Patients and Healthy Individuals

SNPs	Genotype Frequency n (%)			Allele Frequency n (%)
	Major Homozygote	Heterozygote	Minor Homozygote	Major Allele	Minor Allele
rs5029924	CC	CT	TT	C	T
RA (n=123, %)	117 (95.1)	6 (4.9)	0 (0)	240 (97.6)	6 (2.4)
Controls (n=31, %)	28 (90.3)	3 (9.7)	0 (0)	59 (95.2)	3 (4.8)
p-value	-	0.555	-	-	0.561
rs5029937	GG	GT	TT	G	T
RA (n=123, %)	117 (95.1)	6 (4.9)	0 (0)	240 (97.6)	6 (2.4)
Controls (n=31, %)	28 (90.3)	3 (9.7)	0 (0)	59 (95.2)	3 (4.8)
p-value	-	0.555	-	-	0.561
rs2230926	TT	TG	GG	T	G
RA (n=123, %)	117 (95.1)	6 (4.9)	0 (0)	240 (97.6)	6 (2.4)
Controls (n=31, %)	28 (90.3)	3 (9.7)	0 (0)	59 (95.2)	3 (4.8)
p-value	-	0.555	-	-	0.561

Abbreviation: SNPs, nucleotide polymorphisms.

Table 2 Clinical Characteristics of RA Patients Heterozygous for rs5029924, rs2230926 and rs5029937

Patients	Gender	Age(y)	Treatment	Prognosis
P1	Male	72	LEF, MTX	Joints deformities, poor response to DMARDs and died from lung cancer
P2	Female	63	LEF, MTX	Joints deformities, poor response to DMARDs
P3	Female	52	LEF, MTX, Etanercept	Joints deformities, poor response to DMARDs and Etanercept
P4	Female	46	LEF, MTX, Etanercept	Joints deformities, poor response to DMARDs and Etanercept.
P5	Female	54	LEF, MTX, Infliximab, Tocilizumab	Poor response to DMARDs, Infliximab, and Tocilizumab
P6	Female	51	LEF, MTX,	Joints deformities, poor response to DMARDs

Abbreviations: DMARDs, Disease modifying anti-rheumatic drugs; LEF, leflunomide; MTX: methotrexate.

Data Sharing Statement

All supporting data are included in the manuscript and supplemental files. Additional data are available upon reasonable request to the corresponding author.