152
Views
6
CrossRef citations to date
0
Altmetric
Original Research

Carbon nanotube composites for glucose biosensor incorporated with reverse iontophoresis function for noninvasive glucose monitoring

, , , , , , & show all
Pages 343-349 | Published online: 04 May 2010

Figures & data

Figure 1 Construction steps for the planar configuration of the screen-printed transducer. a) (PETE) support material; b) conducting silver basal track; c) insulation layer; d) Ag/AgCl pads, the iontophoresis electrode (the central circular one) for reverse iontophoresis and the reference electrode; e) graphite pads, the working and counter electrodes.

Figure 1 Construction steps for the planar configuration of the screen-printed transducer. a) (PETE) support material; b) conducting silver basal track; c) insulation layer; d) Ag/AgCl pads, the iontophoresis electrode (the central circular one) for reverse iontophoresis and the reference electrode; e) graphite pads, the working and counter electrodes.

Figure 2 Hydrodynamic voltammogram of the biosensors in constantly-stirred solution of 4 mM glucose with 0.1 M phosphate buffer (pH 7.0).

Abbreviations: GOD, glucose oxidase; MWCNT, multiwalled carbon nanotubes.

Figure 2 Hydrodynamic voltammogram of the biosensors in constantly-stirred solution of 4 mM glucose with 0.1 M phosphate buffer (pH 7.0).Abbreviations: GOD, glucose oxidase; MWCNT, multiwalled carbon nanotubes.

Figure 3 Current-time curve obtained by successive addition of 4 mM glucose solution to the biosensor. Data is obtained from biosensor with the combination of MWCNT (18.0% w/w) and GOD (2.0% w/w).

Abbreviations: GOD, glucose oxidase; MWCNT, multiwalled carbon nanotubes.

Figure 3 Current-time curve obtained by successive addition of 4 mM glucose solution to the biosensor. Data is obtained from biosensor with the combination of MWCNT (18.0% w/w) and GOD (2.0% w/w).Abbreviations: GOD, glucose oxidase; MWCNT, multiwalled carbon nanotubes.

Figure 4 Calibration curves of the biosensors to glucose concentration. The lines are best fit found by linear regression. Sensitivity of the biosensor is indicated by the slope of the linear regression line.

Abbreviations: GOD, glucose oxidase; MWCNT, multiwalled carbon nanotubes.

Figure 4 Calibration curves of the biosensors to glucose concentration. The lines are best fit found by linear regression. Sensitivity of the biosensor is indicated by the slope of the linear regression line.Abbreviations: GOD, glucose oxidase; MWCNT, multiwalled carbon nanotubes.

Table 1 The sensitivity (n = 5) of the biosensor at different combinations of glucose oxidase (GOD) loading and multiwalled carbon nanotubes (MWCNT) loading

Figure 5 Evaluation of the biosensor for noninvasive glucose measurement by reverse iontophoresis. An excellent linear relationship (r2 = 0.986) between the biosensor current response and glucose concentration in diffusion cell was found. Data (n = 5) was obtained from biosensor with the optimum combination of MWCNT (18.0% w/w) and GOD (2.0% w/w). The diffusion cell was filled with an electrolyte solution comprising 0.1 M phosphate buffer (pH 7.0) and 3–15mM glucose.

Abbreviations: GOD, glucose oxidase; MWCNT, multiwalled carbon nanotubes.

Figure 5 Evaluation of the biosensor for noninvasive glucose measurement by reverse iontophoresis. An excellent linear relationship (r2 = 0.986) between the biosensor current response and glucose concentration in diffusion cell was found. Data (n = 5) was obtained from biosensor with the optimum combination of MWCNT (18.0% w/w) and GOD (2.0% w/w). The diffusion cell was filled with an electrolyte solution comprising 0.1 M phosphate buffer (pH 7.0) and 3–15mM glucose.Abbreviations: GOD, glucose oxidase; MWCNT, multiwalled carbon nanotubes.