Figures & data
Figure 1 This material crystallizes with the face-centered cubic unit cell typical of spinel 3d metal oxides.
Notes: Their crystallite size, according to XRD and TEM images, remains below 12 nm (see ). The IR spectrum of citric acid-coated nanoparticles is conclusive regarding the anchoring of the organic molecule on the nanoparticles’ surface. (A–C) HR-TEM micrographs at different resolutions from manganese ferrite nanocrystals. (D) XRD pattern. (E) IR spectrum of Mn-ferrite nanoparticles under study.
Abbreviations: IR, infrared; MNPs, magnetic nanoparticles; HR-TEM, high resolution transmission electron microscopy; XRD, X-ray diffraction.
Figure 2 Candida albicans growth curves in RPMI-1640 medium using different concentrations of modified MnFe2O4-NPs in a final volume of 200 µL/well.
Notes: Each value represents the mean of three replicates in three different experiments. Bars represent the standard deviation.
Abbreviations: MNPs, magnetic nanoparticles; OD, optical density; RPMI, Roswell Park Memorial Institute.
Figure 3 Viability and proliferation of the RAW 264.7 cells in RPMI-1640 medium at different concentrations of citric acid-modified MnFe2O4-NPs.
Notes: Triton X-100 (3%) was used as a positive control for toxicity, and PBS was used as a negative control. Each column represents the mean of three replicates. Bars represent the standard deviation.
Abbreviations: MnFe2O4-NPs, manganese ferrite nanoparticles; NPs, nanoparticles; OD, optical density; PBS, phosphate-buffered saline; RPMI, Roswell Park Memorial Institute.
Figure 4 Interaction of modified MnFe2O4-NPs with Candida albicans cells visualized by optical microscopy.
Notes: (A) C. albicans cells (105 cells/mL) in RPMI-1640 medium at 30°C. (B) C. albicans cells (105 cells/mL) in RPMI-1640 medium at 30°C with citric acid-modified MnFe2O4-NPs (250 µg/mL).
Abbreviations: MnFe2O4-NPs, manganese ferrite nanoparticles; RPMI, Roswell Park Memorial Institute.
Figure 5 Antifungal activity assays.
Notes: (A) Candida albicans growth curves in RPMI-1640 medium of modified MnFe2O4-NPs and Cm-p5-conjugated MnFe2O4-NPs. Each value represents the mean of three replicates in three different experiments. Bars represent the standard deviation. (B) C. albicans growth curves in RPMI-1640 medium of the Cm-p5 peptide and Cm-p5-conjugated MnFe2O4-NPs. Each value represents the mean of three replicates in three different experiments. Bars represent the standard deviation.
Abbreviations: MNPs, magnetic nanoparticles; MnFe2O4-NPs, manganese ferrite nanoparticles; OD, optical density; RPMI, Roswell Park Memorial Institute.
