Figures & data
Figure 1 TEM image of nanoparticles manufactured by electric non-explosive method: a) gold b) platinum.
![Figure 1 TEM image of nanoparticles manufactured by electric non-explosive method: a) gold b) platinum.](/cms/asset/d3f2863e-09e7-4f5c-8be2-6712fac0a2a0/dijn_a_12184690_f0001_b.jpg)
Figure 2 TEM image of Salmonella Enteritidis and gold (Au) nanoparticles: a) control; b, c, and d) Salmonella Enteritidis with Au nanoparticles. Arrows point to nano-Au.
![Figure 2 TEM image of Salmonella Enteritidis and gold (Au) nanoparticles: a) control; b, c, and d) Salmonella Enteritidis with Au nanoparticles. Arrows point to nano-Au.](/cms/asset/9c462bc9-3963-422b-9054-e6fafcd69b12/dijn_a_12184690_f0002_b.jpg)
Figure 3 TEM image of Listeria monocytogenes and gold (Au) nanoparticles: a) control; b, c, and d) Listeria monocytogenes with Au nanoparticles. Arrows point to nano-Au (b and d); and to aglomerate of nano-Au (c).
![Figure 3 TEM image of Listeria monocytogenes and gold (Au) nanoparticles: a) control; b, c, and d) Listeria monocytogenes with Au nanoparticles. Arrows point to nano-Au (b and d); and to aglomerate of nano-Au (c).](/cms/asset/cad05fdf-3841-4ce1-9b6e-e620e9c77a18/dijn_a_12184690_f0003_b.jpg)
Figure 4 TEM image of Salmonella Enteritidis and platinum (Pt) nanoparticles: a) control; b, c, and d) the same cell of Salmonella Enteritidis with Pt nanoparticles. Arrows point to nano-Pt (b); and the spots observed with increasing magnification (b, c, and d). Black spots are seen evaporating under the microscope electron beam and are finally seen as white spots.
![Figure 4 TEM image of Salmonella Enteritidis and platinum (Pt) nanoparticles: a) control; b, c, and d) the same cell of Salmonella Enteritidis with Pt nanoparticles. Arrows point to nano-Pt (b); and the spots observed with increasing magnification (b, c, and d). Black spots are seen evaporating under the microscope electron beam and are finally seen as white spots.](/cms/asset/f1d8c32c-8644-41cd-be75-d7b55d245c71/dijn_a_12184690_f0004_b.jpg)
Figure 5 TEM image of Listeria monocytogenes and platinum (Pt) nanoparticles: a) control; b, c, and d) Listeria monocytogenes with nano-Pt. Arrows point to nano-PT (b and c); and to black spot partially evaporated under microscope electron beam (b).
![Figure 5 TEM image of Listeria monocytogenes and platinum (Pt) nanoparticles: a) control; b, c, and d) Listeria monocytogenes with nano-Pt. Arrows point to nano-PT (b and c); and to black spot partially evaporated under microscope electron beam (b).](/cms/asset/10d82635-3431-4e16-9177-461f8fbec6a2/dijn_a_12184690_f0005_b.jpg)
Figure 6 TEM image of Salmonella Enteritidis and platinum (Pt) nanoparticles, after washing and centrifugation to remove nanoparticles unattached to bacteria: a) control; b, c, and d) Salmonella Enteritidis with nano-Pt. Arrows point to nano-Pt complexes.
![Figure 6 TEM image of Salmonella Enteritidis and platinum (Pt) nanoparticles, after washing and centrifugation to remove nanoparticles unattached to bacteria: a) control; b, c, and d) Salmonella Enteritidis with nano-Pt. Arrows point to nano-Pt complexes.](/cms/asset/752434c5-8633-4660-a83c-396ec11977b3/dijn_a_12184690_f0006_b.jpg)
Figure 7 Confocal microscope image of Salmonella Enteritidis: 1) control; 2, 3, and 4) after incubation with platinum nanoparticles for 45, 60, and 90 min respectively. a) Nomarski contrast; b) stained with DAPI; and c) merged pictures.
![Figure 7 Confocal microscope image of Salmonella Enteritidis: 1) control; 2, 3, and 4) after incubation with platinum nanoparticles for 45, 60, and 90 min respectively. a) Nomarski contrast; b) stained with DAPI; and c) merged pictures.](/cms/asset/ac45b5d2-aeaa-417e-94cb-2b6e16da6d71/dijn_a_12184690_f0007_b.jpg)
Table 1 Average number and viability of Salmonella Enteritidis incubated with nano-Pt for 45, 60, and 90 min. DNA visualization was performed by labelling bacteria with DAPI (DNA-binding fluorochrome)