Figures & data
Figure 1 A) Determination of folate contents in PTX-FA-BSANPs. B) Physical status of paclitaxel and freeze-dried PTX-FA-BSANPs.
Abbreviation: PTX-FA-BSANPs, paclitaxel-loaded biodegradable bovine serum albumin nanoparticles with folate decoration.
Figure 2 Characterization of PTX-BSANPs and PTX-FA-BSANPs. Scanning electron microscopic images of A) PTX-BSANPs. B) PTX-BSANPs with mannitol. D) PTX-FA-BSANPs. E) PTX-FA-BSANPs with mannitol; particle polydispersity of C). PTX-BSANPs. F) PTX-FA-BSANPs obtained from laser light scattering.
Abbreviations: PTX-BSANPs, paclitaxel-loaded biodegradable bovine serum albumin nanoparticles; PTX-FA-BSANPs, paclitaxel-loaded biodegradable bovine serum albumin nanoparticles with folate decoration.
Figure 3 A) Freeze-dried powder. B) Reconstitution appearance of PTX-FA-BSANP solution.
Abbreviation: PTX-FA-BSANP, paclitaxel-loaded biodegradable bovine serum albumin nanoparticles with folate decoration.
Figure 4 In vitro paclitaxel release profile from PTX-FA-BSANPs. Phosphate-buffered saline (0.1 M, pH = 7.4) was selected as the release medium. The nanoparticle dispersion was put in an orbital shaker and shaken at 120 rpm at 37°C. High performance liquid chromatography was performed to measure the released drug concentration.
Abbreviation: PTX-FA-BSANP, paclitaxel-loaded biodegradable bovine serum albumin nanoparticles with folate decoration.
Figure 5 Confocal laser scanning microcopy images show the internalization of fluorescent nanoparticles in cells. A) Prostate cancer cells with no drug incubation. B) Prostate cancer cells incubated with PTX-BSANPs for four hours. C) Prostate cancer cells incubated with PTX-FA-BSANPs for four hours. D) Prostate cancer cells incubated with PTX-FA-BSANPs for 12 hours.
Abbreviations: PTX-BSANP, paclitaxel-loaded biodegradable bovine serum albumin nanoparticles; PTX-FA-BSANP, paclitaxel-loaded biodegradable bovine serum albumin nanoparticles with folate decoration.
Figure S1 Chromatograph profile of paclitaxel by high performance liquid chromatography.
