Magnetic Fe₃O₄ nanoparticles and chemotherapy agents interact synergistically to induce apoptosis in lymphoma cells

Figures & data

Figure 1 Proliferation inhibitory ratio of Raji cells incubated with different drugs with or without MNP-Fe₃O₄. A) ADM incubated with Raji cells for 48 hours. B) DNR incubated with Raji cells for 48 hours. C) Either 0.1 μg/mL ADM or 0.1 μg/mL DNR ± 50 μmol/L MNP-Fe₃O₄ incubated with Raji cells for different lengths of time.

Abbreviations: MNP-Fe₃O₄, magnetic nanoparticles of Fe₃O₄; ADM, adriamycin; DNR, daunorubicin.

Table 1 Cell viability of different drugs with or without MNP-Fe₃O₄ for different times (%, mean ± SD)

Group	6 hours	12 hours	24 hours	48 hours	72 hours
0.1 μg/mL ADM	92 ± 0.3	88.3 ± 0.6	82.2 ± 0.6	78.3 ± 0.6	70.8 ± 0.5
0.1 μg/mL ADM + 50 μmol/L MNP-Fe3O4	91.4 ± 0.7	86.4 ± 0.7	80.3 ± 0.9	76.4 ± 0.5	66.7 ± 1.1
0.1 μg/mL DNR	92.6 ± 0.4	87.3 ± 0.6	81.1 ± 0.2	79.1 ± 0.7	69.6 ± 1.0
0.1 μg/mL DNR + 50 μmol/LMNP-Fe3O4	91.6 ± 0.3	85.2 ± 0.5	80.0 ± 0.7	77.4 ± 0.9	64.5 ± 2.3
100 μg/mL DEX	93.0 ± 0.5	90.1 ± 0.4	84.5 ± 0.9	79.8 ± 0.8	73.1 ± 0.8
100 μg/mL DEX + 50 μmol/L MNP-Fe3O4	92.4 ± 0.3	89.6 ± 0.8	85.7 ± 0.7	80.9 ± 1.0	71.5 ± 1.2

Abbreviations: MNP-Fe₃O₄, magnetic nanoparticles of Fe₃O₄; ADM, adriamycin; DNR, daunorubicin; DEX, dexamethasone.

Figure 2 Apoptosis of Raji cells incubated with different drugs for 48 hours. A) control, B) MNP-Fe₃O₄, C) ADM, D) ADM + MNP-Fe₃O₄, E) DNR, F) DNR + MNP-Fe₃O₄, G) DEX, H) DEX + MNP-Fe₃O₄.

Abbreviations: MNP-Fe₃O₄, magnetic nanoparticles of Fe₃O₄; ADM, adriamycin; DNR, daunorubicin; DEX, dexamethasone.

Figure 3 Expressions of P53 and NF-κB protein in Raji cells. Lane 1) control; Lane 2) 0.1 μg/mL ADM; Lane 3) 0.1 μg/mL DNR; Lane 4) 100 μg/mL DEX; Lane 5) 50 μmol/L MNP-Fe₃O₄; Lane 6) 0.1 μg/mL ADM+ 50 μmol/L MNP-Fe₃O₄; Lane 7) 0.1μg/mL DNR+ 50 μmol/L MNP-Fe₃O₄; Lane 8) 100 μg/mL DEX+ 50 μmol/L MNP-Fe₃O₄.

Abbreviations: MNPs(Fe₃O₄), magnetic nanoparticles of Fe₃O₄; ADM, adriamycin; DNR, daunorubicin; DEX, dexamethasone.