In vitro and in vivo study of a nanoliposomal cisplatin as a radiosensitizer

Figures & data

Figure 1 Effect of free liposomes, NLE-CDDP, and CDDP on A549 cells.

Abbreviations: CDDP, cisplatin; NLE-CDDP, nanoliposome encapsulated cisplatin.

Figure 2 Cell survival curves after treatments with radiation alone (R) or combined with 0.5 μg/mL NLE-CDDP (NLE-CDDP + R) or CDDP (CDDP + R) (P = 0.00 for CDDP vs R; P = 0.00 for NLE-CDDP vs R; P = 0.043 for CDDP vs NLE-CDDP).

Abbreviations: CDDP, cisplatin; NLE-CDDP, nanoliposome encapsulated cisplatin.

Table 1 Tumor growth delay in Lewis lung carcinoma after NLE-CDDP plus 6 Gy or CDDP plus 6 Gy with different intervals

Group	T5V0 (days)	TGD (days)
Control	6.12	0
6 Gy	7.95	1.83
NLE-CDDP	18.07	11.95
CDDP	9.39	3.27
NLE-CDDP-1 h-6 Gy	18.33	12.21
NLE-CDDP-24 h-6 Gy	19.16	13.04
NLE-CDDP-72 h-6 Gy	26.97	20.85
CDDP-1 h-6 Gy	10.85	4.73
CDDP-24 h-6 Gy	11.11	4.99
CDDP-72 h-6 Gy	12.77	6.65

Abbreviations: CDDP, cisplatin; NLE-CDDP, nanoliposome encapsulated cisplatin; TGD, tumor growth delay; T_5V0, the days needed for tumor growth from the original size to five times the original volume.

Figure 3 Tumor growth curves after irradiation of 6 Gy, 6 mg/kg of NLE-CDDP or CDDP or combinations of 6 Gy and NLE-CDDP/CDDP. A) Tumors treated with 6 Gy, NLE-CDDP, CDDP, or combinations of 6 Gy and NLE-CDDP/CDDP with 1 h interval between drugs and irradiation (P = 0.000). B) Tumors treated with 6 Gy, NLE-CDDP, CDDP, or combinations of 6 Gy and NLE-CDDP/CDDP with 24 h interval between drugs and irradiation (P = 0.000). (P = 0.001). C) Tumors treated with 6 Gy, NLE-CDDP, CDDP, or combinations of 6 Gy and NLE-CDDP/CDDP with 72 h interval between drugs and irradiation (P = 0.000). (P = 0.0003). (Relative tumor volume: a ratio of tumor volume after treatment versus the pretreatment volume.)

Abbreviations: CDDP, cisplatin; NLE-CDDP, nanoliposome encapsulated cisplatin.

Table 2 Tumor growth delay (days) in Lewis lung carcinoma after NLE-CDDP plus irradiation, or CDDP plus irradiation with 72 h interval

Group	0 Gy	2 Gy	6 Gy	16 Gy	28 Gy
Irradiation-alone	0	0.4	1.83	1.99	7.48
NLE-CDDP + irradiation	11.95	15.29	20.85	19.54	56.88
CDDP + irradiation	3.27	3.96	6.65	13.38	21.98

Abbreviations: CDDP, cisplatin; NLE-CDDP, nanoliposome encapsulated cisplatin.

Figure 4 Tumor growth curves treated with a single dose of irradiation, NLE-CDDP plus irradiation, or CDDP plus irradiation with 72 h interval. A) Tumor treated with single doses of irradiation. B) Tumor treated with 6 mg/kg of NLE-CDDP followed by irradiation. C) Tumor treated with 6 mg/kg of CDDP followed by irradiation.

Abbreviations: CDDP, cis-platinum diammine dichloride; NLE-CDDP, nanoliposome encapsulated cisplatin.

Figure 5 Dose-response curves from irradiation combined with NLE-CDDP or CDDP administered 72 h prior to irradiation in Lewis lung carcinoma.

Abbreviations: CDDP, cis-platinum diammine dichloride; NLE-CDDP, nanoliposome encapsulated cisplatin; R, radiation alone.

Figure 6 Jejunal crypt cell survivals after irradiation (R), combinations of CDDP and irradiation (CDDP + R), or NLE-CDDP and irradiation (NLE-CDDP + R) with 72 h interval. L-Q model: Irradiation alone: y = exp (0.18*d − 0.026*d²), R² = 1.000 NLE-CDDP + irradiation: y = exp(0.202*d − 0.03*d²), R² = 0.980 CDDP + irradiation: y = exp(0.218*d − 0.031*d²), R² = 0.999.

Abbreviations: CDDP, cis-platinum diammine dichloride; NLE-CDDP, nanoliposome encapsulated cisplatin; R, irradiation-alone.