Novel formulation and evaluation of a Q10-loaded solid lipid nanoparticle cream: in vitro and in vivo studies

Figures & data

Table 1 The best formulation of solid lipid nanoparticles when the percentage of solid lipid changes

	Ingredient	Weight/weight percentage	The best formulation
Oil phase	Solid lipid	10–30	13
	Coenzyme Q10	0.01	0.01
Water phase	Hydrophilic surfactant	13	13
	Distilled water	57–77	74

Table 2 The best formulation of solid lipid nanoparticles when the percentage of surfactant changes

	Ingredient	Weight/weight percentage	The best formulation
Oil phase	Solid lipid	13	13
	Coenzyme Q10	0.01	0.01
Water phase	Hydrophilic surfactant	8–18	8
	Distilled water	69–79	79

Table 3 The best formulations of solid lipid nanoparticles (SLNs) with suitable stability and particle size

No.	Solid lipid	Surfactant	Water phase	Active ingredient	Particle size (nm)	Zeta potential (mV)	Polydispersity index
SLN1	Cetyl palmitate	Tegocare 450	Water	Q10	100 ± 15	–44.4	0.203
SLN2	Stearic Acid	Tween 80	Water	Q10	50 ± 10	–47.1	0.109
Percentage of above ingredients	13	8	79	0.01

Table 4 Ingredients used in formulation of simple cream and cream containing solid lipid nanoparticles

	Solid lipid nanoparticle	Simple cream
Oil phase	Stearic acid 13%	Cetyl alcohol 1%
		Stearyl alcohol 1%
		Cetyl palmitate 1%
		Isopropyl myristate 10%
Water phase	Distilled water 79%	Distilled water 82%
	Tween 80 8%	Tween 80 3.5%
		Span 60 1.5%
Active ingredient	Coenzyme Q10 0.02%

Figure 1 Transmission electron microscopy photograph of a Q10-loaded solid lipid nanoparticle.

Figure 2 Elevation of lipid content in formulation increases the particle size.

Figure 3 Differential scanning calorimetry thermogram of sample A (stearic acid), sample B (coenzyme Q10), sample C (solid lipid nanoparticle), and sample D (Q10-loaded solid lipid nanoparticle).

Figure 4 Concentration of released Q10 versus time for simple Q10, solid lipid nanoparticle (SLN), and Q10-loaded SLN cream.

Figure 5 Percentage of released Q10 versus time for simple Q10, solid lipid nanoparticle (SLN), and Q10-loaded SLN cream.

Figure 6 Percentage of increase in hydration for simple cream and solid lipid nanoparticle (SLN) cream containing Q10 after 1- and 2-month application periods. All results are expressed as values related to the measured values on day 0 and the untreated control (mean ± standard deviation, n = 25; P < 0.05).

Figure 7 Percentage of increase in elasticity for simple and solid lipid nanoparticle (SLN) cream containing Q10 after 1- and 2-month application periods. All results are expressed as values related to the measured values on day 0 and the untreated control (mean ± standard deviation, n = 25; P < 0.05).

Figure 8 Changes in skin pH after using solid lipid nanoparticle (SLN) cream and simple cream (mean ± standard deviation, n = 25; P < 0.05).