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International Journal of Nanomedicine Volume 6, 2011 - Issue
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Original Research

Effects of an antibacterial membrane on osteoblast-like cells in vitro

Jun Ye1 State Key Laboratory of Oral Diseases, Sichuan University, Chengdu, People’s Republic of China
,
Qianqian Yao1 State Key Laboratory of Oral Diseases, Sichuan University, Chengdu, People’s Republic of China
,
Anchun Mo2 Department of Oral Implant, West China College of Stomatology, Sichuan University, Chengdu, People’s Republic of ChinaCorrespondence[email protected]
,
Jing Nie2 Department of Oral Implant, West China College of Stomatology, Sichuan University, Chengdu, People’s Republic of China
,
Wenwen Liu1 State Key Laboratory of Oral Diseases, Sichuan University, Chengdu, People’s Republic of China
,
Cui Ye1 State Key Laboratory of Oral Diseases, Sichuan University, Chengdu, People’s Republic of China
&
Xianji Chen1 State Key Laboratory of Oral Diseases, Sichuan University, Chengdu, People’s Republic of China
 show all
Pages 1853-1861 | Published online: 06 Sep 2011

Figures & data

Figure 1 (A) Microscopic section of the Ag-nHA-nTiO2/PA66 (AM) membrane (H&E ×100); (B) microscopic section of the nHA/PA66 membrane (H&E ×100); (C) microscopic section of the e-PTFE membrane (H&E ×200).

Notes: +, the observe; –, the reverse.

Abbreviations: e-PTFE, expanded poly tetrafluroethylene; H&E, hematoxylin and eosin stain; PA66, polyamide-66.

Figure 1 (A) Microscopic section of the Ag-nHA-nTiO2/PA66 (AM) membrane (H&E ×100); (B) microscopic section of the nHA/PA66 membrane (H&E ×100); (C) microscopic section of the e-PTFE membrane (H&E ×200).Notes: +, the observe; –, the reverse.Abbreviations: e-PTFE, expanded poly tetrafluroethylene; H&E, hematoxylin and eosin stain; PA66, polyamide-66.

Figure 2 (A) SEM image of surface of obverse of the Ag-nHA-nTiO2/PA66 membrane (×500); (B) SEM image of surface of reverse of the Ag-nHA-nTiO2/PA66 membrane (×500); (C) SEM image of surface of obverse of the e-PTFE membrane (×2000); (D) SEM image of surface of reverse of the e-PTFE membrane (×2000).

Abbreviations: e-PTFE, expanded poly tetrafluroethylene; PA66, polyamide-66; SEM, scanning electronic microscope.

Figure 2 (A) SEM image of surface of obverse of the Ag-nHA-nTiO2/PA66 membrane (×500); (B) SEM image of surface of reverse of the Ag-nHA-nTiO2/PA66 membrane (×500); (C) SEM image of surface of obverse of the e-PTFE membrane (×2000); (D) SEM image of surface of reverse of the e-PTFE membrane (×2000).Abbreviations: e-PTFE, expanded poly tetrafluroethylene; PA66, polyamide-66; SEM, scanning electronic microscope.

Table 1 MTT values of experimental and control groups at different times

Table 2 Mean CVA values of MG63 osteoblast-like cells on experimental and control groups

Table 3 Alkaline phosphatase activity at 1, 3, 5, and 7 days in experimental and control groups

Table 4 Ca2+ concentration at 1, 3, 5, and 7 days in experimental and control groups

Figure 3 (A and B) Adhesion of MG63 osteoblast-like cells on day 1 on the Ag- nHA-nTiO2/PA66 membrane (SEM * 400)/(SEM * 1000). (C and D) Adhesion of MG63 osteoblast-like cells on day 5 day on the Ag-nHA-nTiO2/PA66 membrane (SEM * 400)/(SEM * 1000).

Abbreviations: PA66, polyamide-66; SEM, scanning electronic microscope.

Figure 3 (A and B) Adhesion of MG63 osteoblast-like cells on day 1 on the Ag- nHA-nTiO2/PA66 membrane (SEM * 400)/(SEM * 1000). (C and D) Adhesion of MG63 osteoblast-like cells on day 5 day on the Ag-nHA-nTiO2/PA66 membrane (SEM * 400)/(SEM * 1000).Abbreviations: PA66, polyamide-66; SEM, scanning electronic microscope.

Figure 4 (A and B) Adhesion of MG63 osteoblast-like cells on day 1 on the nHA/PA66 membrane (SEM * 400)/(SEM * 1000). (C and D) Adhesion of MG63 osteoblast-like cells on day 5 on the nHA/PA66 membrane (SEM * 400)/(SEM * 1000).

Abbreviations: PA66, polyamide-66; SEM, scanning electronic microscope.

Figure 4 (A and B) Adhesion of MG63 osteoblast-like cells on day 1 on the nHA/PA66 membrane (SEM * 400)/(SEM * 1000). (C and D) Adhesion of MG63 osteoblast-like cells on day 5 on the nHA/PA66 membrane (SEM * 400)/(SEM * 1000).Abbreviations: PA66, polyamide-66; SEM, scanning electronic microscope.

Figure 5 (A and B) Adhesion of MG63 osteoblast-like cells on day 1 on the e-PTFE membrane (SEM * 400)/(SEM * 1000). (C and D) Attachment of MG63 osteoblastlike cells on day 5 day on the e-PTFE membrane (SEM * 400)/(SEM * 1000).

Abbreviations: e-PTFE, expanded poly tetrafluroethylene; SEM, scanning electronic microscope.

Figure 5 (A and B) Adhesion of MG63 osteoblast-like cells on day 1 on the e-PTFE membrane (SEM * 400)/(SEM * 1000). (C and D) Attachment of MG63 osteoblastlike cells on day 5 day on the e-PTFE membrane (SEM * 400)/(SEM * 1000).Abbreviations: e-PTFE, expanded poly tetrafluroethylene; SEM, scanning electronic microscope.

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