Figures & data
Figure 1 In vivo SELEX of bone targeting aptamer in nude mice bearing PC3 PB.
Notes: (A) Bioluminescence produced by luciferase in nude mice with PC3 PB (left) and bone marrow tumor sites stained using H&E (right). (B) In vivo SELEX procedures. (C) The three aptamers with the highest hitting points after SELEX.
Figure 2 Higher accumulation of the PB aptamer in bone of tumor mouse compared with the control aptamer, more accumulation of the PB aptamer in bone of tumor mice compared with healthy mice (*P<0.05).
Notes: (A) Distribution of the PB aptamer and control aptamer in different organs ex vivo. (B) Fluorescence intensity of aptamer in different organs.
Figure 3 (A) Distribution of the PB aptamer relative to bone marrow vascular endothelium (red: Cy5-PB aptamer; blue: nuclei). (B) Colocalization of PB aptamer with a vascular endothelial cell marker in bone marrow (red: Cy5-PB aptamer; green: CD31; blue: nuclei). (C) Colocalization of PB aptamer with a vascular endothelial cell marker within tumor in bone marrow (red: Cy5-PB aptamer; green: CD31; blue: nuclei). (D) Higher binding affinity of PB aptamer to bone marrow endothelial cell compared with control aptamer ex vivo (*P<0.05).
Figure 4 (A) Increased PB aptamer uptake in HUVEC grown in PC3 prostate cancer cell-conditioned medium under fluorescence microscopy (red: Cy5-PB aptamer; green: actin; blue: nuclei). Scale bar: 20 μm. (B) Increased PB aptamer uptake in HUVECs grown in PC3 prostate cancer cell-conditioned medium (P<0.05). (C) Increased PB aptamer uptake in HUVECs grown in bone marrow with PC3 or DU145 prostate cancer cell-conditioned medium, while decreased PB aptamer uptake in HUVECs grown in bone marrow with LNCaP prostate cancer cell-conditioned medium (*P<0.05).
Abbreviation: HUVECs, human umbilical vein endothelial cells.
Figure 5 (A) Decreased PB aptamer uptake by chlorpromazine (clathrin-mediated endocytosis inhibitor) and dynasore (dynamin-mediated endocytosis inhibitor) treated HUVEC (*P<0.05). (B) Escape of the PB aptamer from lysosome 6 hours after HUVEC internalization (red: Cy5-PB aptamer; green: lysosome; blue: nuclei). Scale bar: 20 μm.
Abbreviation: HUVEC, human umbilical vein endothelial cell.
Figure 6 (A) Cy5-PB-Au particle under fluorescence microscopy. (B) Accumulation of a few Cy5-Au particles within tumor in bone marrow and marked accumulation of Cy5-PB-Au particle within tumor in bone marrow (red: Cy5-Au/Cy5-PB-Au particle; blue: nuclei) (*P<0.05). (C) Colocalization of Cy5-PB-Au particle with a vascular endothelial cell marker within tumor in bone marrow (red: Cy5-PB-Au particle; green: CD31; blue: nuclei).
