Figures & data
Table 1 Primer sequences used for RT-PCR
Figure 1 Structural property of Gd-BG scaffold.
Notes: (A) SEM image and (B) TEM image of Gd-BGS microspheres; (C) nitrogen adsorption–desorption isotherm, and (D) BJH pore size distribution curve of mesoporous Gd-BGS microspheres. (E) The X-ray diffraction patterns of samples: (I) Gd-BG microspheres and (II) Gd-BG scaffolds. (F) The Fourier transform infrared spectra of samples: (I) Gd-BG microspheres and (II) Gd-BG scaffolds.
Abbreviations: BJH, Barrett–Joyner–Halenda; Gd-BG, gadolinium-doped bioglass; SEM, scanning electron microscopy; TEM, transmission electron microscopy.
![Figure 1 Structural property of Gd-BG scaffold.Notes: (A) SEM image and (B) TEM image of Gd-BGS microspheres; (C) nitrogen adsorption–desorption isotherm, and (D) BJH pore size distribution curve of mesoporous Gd-BGS microspheres. (E) The X-ray diffraction patterns of samples: (I) Gd-BG microspheres and (II) Gd-BG scaffolds. (F) The Fourier transform infrared spectra of samples: (I) Gd-BG microspheres and (II) Gd-BG scaffolds.Abbreviations: BJH, Barrett–Joyner–Halenda; Gd-BG, gadolinium-doped bioglass; SEM, scanning electron microscopy; TEM, transmission electron microscopy.](/cms/asset/8cd0d71e-f603-4b8d-8b72-f31a8399a38a/dijn_a_12190755_f0001_c.jpg)
Figure 2 Morphology and chemical composition.
Notes: Characterization of Gd-BG scaffolds: (A) low-resolution SEM image; (B) high-resolution SEM image; (C) Ca element distribution map; (D) Si element distribution map; (E) Gd element distribution map; and (F) energy-dispersive X-ray spectrometry pattern.
Abbreviations: Gd-BG, gadolinium-doped bioglass; SEM, scanning electron microscopy.
![Figure 2 Morphology and chemical composition.Notes: Characterization of Gd-BG scaffolds: (A) low-resolution SEM image; (B) high-resolution SEM image; (C) Ca element distribution map; (D) Si element distribution map; (E) Gd element distribution map; and (F) energy-dispersive X-ray spectrometry pattern.Abbreviations: Gd-BG, gadolinium-doped bioglass; SEM, scanning electron microscopy.](/cms/asset/f180c57c-27e0-419b-b7aa-97ffe1386069/dijn_a_12190755_f0002_c.jpg)
Figure 3 Cell proliferation and adhesion on BG and Gd-BG scaffolds.
Notes: (A) Viability of hBMSCs at different concentrations of BG and Gd-BG scaffolds. SEM images showing the attachment of hBMSCs on (B) BG and (C) Gd1/3-BG. Data are presented as mean ± SD from a representative of three separate experiments performed in quadruplicate (*P<0.05).
Abbreviations: Gd-BG, gadolinium-doped bioglass; hBMSC, human bone marrow-derived mesenchymal stem cell; SEM, scanning electron microscopy.
![Figure 3 Cell proliferation and adhesion on BG and Gd-BG scaffolds.Notes: (A) Viability of hBMSCs at different concentrations of BG and Gd-BG scaffolds. SEM images showing the attachment of hBMSCs on (B) BG and (C) Gd1/3-BG. Data are presented as mean ± SD from a representative of three separate experiments performed in quadruplicate (*P<0.05).Abbreviations: Gd-BG, gadolinium-doped bioglass; hBMSC, human bone marrow-derived mesenchymal stem cell; SEM, scanning electron microscopy.](/cms/asset/9d88d8f2-6ee9-4aa2-ac90-f3c7ea571020/dijn_a_12190755_f0003_c.jpg)
Figure 4 Osteogenic differentiation of hBMSCs in BG and Gd-BG dissolution.
Notes: (A) Effects of BG and Gd-BG dissolution on the ALP activity of hBMSCs after culturing for 7 days. (B) Osteogenic gene expression of BSP and OCN was detected after hBMSCs were treated with BG and Gd-BG dissolution for 3 days. (C) ALP staining showed osteogenic differentiation of hBMSCs treated with BG and Gd-BG dissolution for 14 days (40×). (D) Alizarin red staining showing the mineralization of hBMSCs treated with BG and Gd-BG dissolution for 21 days. Data are presented as mean ± SD from a representative of three separate experiments performed in quadruplicate (**P<0.01).
Abbreviations: Gd-BG, gadolinium-doped bioglass; hBMSC, human bone marrow-derived mesenchymal stem cell.
![Figure 4 Osteogenic differentiation of hBMSCs in BG and Gd-BG dissolution.Notes: (A) Effects of BG and Gd-BG dissolution on the ALP activity of hBMSCs after culturing for 7 days. (B) Osteogenic gene expression of BSP and OCN was detected after hBMSCs were treated with BG and Gd-BG dissolution for 3 days. (C) ALP staining showed osteogenic differentiation of hBMSCs treated with BG and Gd-BG dissolution for 14 days (40×). (D) Alizarin red staining showing the mineralization of hBMSCs treated with BG and Gd-BG dissolution for 21 days. Data are presented as mean ± SD from a representative of three separate experiments performed in quadruplicate (**P<0.01).Abbreviations: Gd-BG, gadolinium-doped bioglass; hBMSC, human bone marrow-derived mesenchymal stem cell.](/cms/asset/9f68d858-8f7d-41f8-8132-2d9e495b71d6/dijn_a_12190755_f0004_c.jpg)
Figure 5 Activation of Akt/GSK3β pathway by Gd-BG.
Notes: (A) Western blotting analysis of p-Akt, total-Akt, p-GSK3β, and total-GSK3β for hBMSCs after treatment with different concentrations of Gd-BG dissolution for 3 hours. GAPDH was used as the loading control. (B) Western blotting analysis of β-catenin for hBMSCs after treatment with different concentrations of Gd-BG dissolution for 24 hours. (C) Western blotting analysis of p-Akt, total-Akt, p-GSK3β, and total-GSK3β for hBMSCs after treatment with BG, Gd1/3-BG dissolution, and Gd1/3-BG dissolution+ LY294002 for 3 hours. (D) Western blotting analysis of β-catenin for hBMSCs after treatment with BG, Gd1/3-BG dissolution, and Gd1/3-BG dissolution+ LY294002 for 24 hours. (E, F) Analysis of protein expression in hBMSCs. (G) Effects of BG, Gd1/3-BG dissolution, and Gd1/3-BG dissolution+ LY294002 on the ALP activity of hBMSCs after culturing for 7 days. (H) ALP staining showed the osteogenic differentiation of hBMSCs after treatment with BG, Gd1/3-BG dissolution, and Gd1/3-BG dissolution+ LY294002 for 14 days (40×). (I) Alizarin red staining showed the mineralization of hBMSCs after treatment with BG, Gd1/3-BG dissolution, and Gd1/3-BG dissolution+ LY294002 for 21 days. Data are presented as mean ± SD from a representative of three separate experiments performed in quadruplicate (**P<0.01).
Abbreviations: ARS, Alizarin red staining; Gd-BG, gadolinium-doped bioglass; hBMSC, human bone marrow-derived mesenchymal stem cell.
![Figure 5 Activation of Akt/GSK3β pathway by Gd-BG.Notes: (A) Western blotting analysis of p-Akt, total-Akt, p-GSK3β, and total-GSK3β for hBMSCs after treatment with different concentrations of Gd-BG dissolution for 3 hours. GAPDH was used as the loading control. (B) Western blotting analysis of β-catenin for hBMSCs after treatment with different concentrations of Gd-BG dissolution for 24 hours. (C) Western blotting analysis of p-Akt, total-Akt, p-GSK3β, and total-GSK3β for hBMSCs after treatment with BG, Gd1/3-BG dissolution, and Gd1/3-BG dissolution+ LY294002 for 3 hours. (D) Western blotting analysis of β-catenin for hBMSCs after treatment with BG, Gd1/3-BG dissolution, and Gd1/3-BG dissolution+ LY294002 for 24 hours. (E, F) Analysis of protein expression in hBMSCs. (G) Effects of BG, Gd1/3-BG dissolution, and Gd1/3-BG dissolution+ LY294002 on the ALP activity of hBMSCs after culturing for 7 days. (H) ALP staining showed the osteogenic differentiation of hBMSCs after treatment with BG, Gd1/3-BG dissolution, and Gd1/3-BG dissolution+ LY294002 for 14 days (40×). (I) Alizarin red staining showed the mineralization of hBMSCs after treatment with BG, Gd1/3-BG dissolution, and Gd1/3-BG dissolution+ LY294002 for 21 days. Data are presented as mean ± SD from a representative of three separate experiments performed in quadruplicate (**P<0.01).Abbreviations: ARS, Alizarin red staining; Gd-BG, gadolinium-doped bioglass; hBMSC, human bone marrow-derived mesenchymal stem cell.](/cms/asset/a1e80607-45dd-4e31-b1c2-f0e8b4306d75/dijn_a_12190755_f0005_c.jpg)
Figure 6 Micro-CT of rat cranial defects implanted with BG and Gd1/3-BG scaffolds at 8 weeks after implantation.
Notes: (A) The images of reconstruction of micro-CT for the bone regeneration of the defect area at week 8. (B, C) BMD and BV/TV in the defects implanted with the Gd1/3-BG and BG scaffolds. Data are presented as mean ± SD from a representative of ten separate experiments (**P<0.01). (D) New bone formation and mineralization measured histomorphometrically by using fluorochrome labeling analysis in rat cranial defects implanted with Gd1/3-BG and BG scaffolds. White arrow indicates the new bone formation. Alizarin red (red) and calcein (green) were intraperitoneally injected at weeks 4 and 6, respectively.
Abbreviations: BMD, bone mineral density; BV/TV, bone volume to total bone volume; CT, computed tomography; Gd-BG, gadolinium-doped bioglass.
![Figure 6 Micro-CT of rat cranial defects implanted with BG and Gd1/3-BG scaffolds at 8 weeks after implantation.Notes: (A) The images of reconstruction of micro-CT for the bone regeneration of the defect area at week 8. (B, C) BMD and BV/TV in the defects implanted with the Gd1/3-BG and BG scaffolds. Data are presented as mean ± SD from a representative of ten separate experiments (**P<0.01). (D) New bone formation and mineralization measured histomorphometrically by using fluorochrome labeling analysis in rat cranial defects implanted with Gd1/3-BG and BG scaffolds. White arrow indicates the new bone formation. Alizarin red (red) and calcein (green) were intraperitoneally injected at weeks 4 and 6, respectively.Abbreviations: BMD, bone mineral density; BV/TV, bone volume to total bone volume; CT, computed tomography; Gd-BG, gadolinium-doped bioglass.](/cms/asset/b9948450-f38a-4d4f-b578-0872681ab891/dijn_a_12190755_f0006_c.jpg)
Figure 7 Histological analysis of cranial bone defects.
Notes: (A) H&E, (B) VG, and (C) immunohistochemical staining of OCN in the defects implanted with Gd-BG and BG scaffolds at 8 weeks after implantation. Black stars indicate new bone formation. Green star indicates new collagen formation, and red stars indicate positive staining of OCN.
Abbreviation: Gd-BG, gadolinium-doped bioglass; VG, Van Gieson’s.
![Figure 7 Histological analysis of cranial bone defects.Notes: (A) H&E, (B) VG, and (C) immunohistochemical staining of OCN in the defects implanted with Gd-BG and BG scaffolds at 8 weeks after implantation. Black stars indicate new bone formation. Green star indicates new collagen formation, and red stars indicate positive staining of OCN.Abbreviation: Gd-BG, gadolinium-doped bioglass; VG, Van Gieson’s.](/cms/asset/e70b35af-86e3-4eeb-9fd3-a4d0e9043180/dijn_a_12190755_f0007_c.jpg)
Figure 8 Gd and Akt/GSK3β signaling pathway, where Gd could activate Akt.
Notes: Phosphorylated Akt could prevent GSK3β from forming a complex with β-catenin, resulting in the accumulation of β-catenin. Thus, it could promote osteogenic gene expression and enhance osteogenic differentiation.
Abbreviation: Gd, gadolinium.
![Figure 8 Gd and Akt/GSK3β signaling pathway, where Gd could activate Akt.Notes: Phosphorylated Akt could prevent GSK3β from forming a complex with β-catenin, resulting in the accumulation of β-catenin. Thus, it could promote osteogenic gene expression and enhance osteogenic differentiation.Abbreviation: Gd, gadolinium.](/cms/asset/b71f0aec-6637-43e9-a875-70f619b2909d/dijn_a_12190755_f0008_c.jpg)