Figures & data
Figure 1 Size and shape of the nanoscale SHXXT decoction. A) Zeta sizer picture of nanoscale SHXXT showing size distribution in Milli-Q water. B) Field emission scanning electron microscopy picture of nanoscale SHXXT on a solid substrate.
Abbreviation: SHXXT, San-Huang-Xie-Xin-Tang.
Figure 2 Effects of chloroform, microscale SHXXT decoction, and nanoscale SHXXT decoction on A) liver-to-body-weight ratio, B) AST level, and C) ALT level.
Notes: Results are represented as means ± standard error of the mean. *Significant difference from control (P < 0.05).
Abbreviations: SHXXT, San-Huang-Xie-Xin-Tang; ALT, alanine aminotransferase; AST, aspartate aminotransferase.
Figure 3 Liver tissue sections of A) control group, B) chloroform group, C) microscale SHXXT decoction group, and D) nanoscale SHXXT decoction group stained with hematoxylin and eosin.
Abbreviation: SHXXT, San-Huang-Xie-Xin-Tang.
Figure 4 Effects of nanoscale SHXXT decoction on A) cell proliferation and viability of hepatic stellate cells as determined by MTT assay. Concentration-dependent cytotoxic effects were evaluated after 48 hours of incubation. Results are represented as means ± standard error of the mean. B) Expression of GAPDH, MMP-2, and TIMP-1 mRNA in hepatic stellate cells. Line 1, control group; line 2, TGF-β1; line 3, TGFβ1 + microscale SHXXT decoction; line 4, TGF-β1 + nanoscale SHXXT decoction.
Notes: *Significant difference from control (P < 0.05).
Abbreviations: TGF-β1, transforming growth factor-beta1; SHXXT, San-Huang-Xie-Xin-Tang.
