Figures & data
Figure 7 HE staining of liver, spleen, and kidney.
Notes: Three-month-old C57/B6 mice were treated with PBS, PP, TP, or PPT. The liver, spleen, and kidney tissues of every treatment group were harvested for pathologic examination (magnification ×200). The circled areas show the renal glomerulus and the arrow is showing the hepatic nuclei.
Abbreviations: HE, hematoxylin and eosin; PBS, phosphate-buffered saline; PP, l-PAAE-γ-PGA complex; l-PAE, l-phenylalanine ethylester; γ-PGA, poly-γ-glutamic acid; TP, triptolide; PPT, γ-PGA-l-PAE-TP.
Figure 8 TUNEL assay in different tissues. Sections of kidney, spleen, and liver tissues of control, PP, TP, and PPT treatment groups were processed for TUNEL assay in order to detect the apoptosis of kidney, spleen, and liver. Nucleus was stained with DAPI (blue), and the apoptosis was stained with green (magnification ×200).
Abbreviations: PP, l-PAE-γ-PGA complex; l-PAE, l-phenylalanine ethylester; γ-PGA, poly-γ-glutamic acid; TP, triptolide; PPT, γ-PGA-l-PAE-TP; TUNEL, terminal-deoxynucleotidyl dUTP transferase nick-end labeling; DAPI, 4′,6-diamidino-2-phenylindole.
