Improved biocompatibility of novel poly(L-lactic acid)/β-tricalcium phosphate scaffolds prepared by an organic solvent-free method

Figures & data

Table 1 Specific primers designed following the cDNA sequences of each gene in GenBank

Gene	GenBank accession number	Primers	Product size
ALP	NM_013059	(+), 5′-CGTCTCCATGGTGGATTATGCT-3′ (−), 5′-CCAGGCACAGTGGTCAAG-3′	209 bp
OCN	NM_013414	(+), 5′-TACCTCAACAATGGACTTGGAGC-3′ (−), 5′-CTGGAGAGTAGCAAAGCTGAAG-3′	206 bp
COL-1	XM_213440	(+), 5′-GCATGGCAAGAAGACATCC-3′ (−), 5′-CTCCTATGACTTCTGCGTCTGGT-3′	239 bp
β-Actin	NM_031144	(+), 5′-CACGCATTGTCACCAACTG-3′ (−), 5′-TTGAAGGTCTCAAACATGATCTGG-3′	167 bp

Figure 1 SEM images of scaffolds made by PCLP method (A, C) and by conventional organic solvent casting method (B, D).

Abbreviations: PCLP, powder mixing, compression molding, low-temperature treatment, and particulate leaching; SEM, scanning electron microscopy.

Figure 2 Compressive strength of scaffolds.

Notes: ^aP < 0.05; n = 6.

Figure 3 SEM images of osteoblasts cultured on the scaffolds for 1 day: (A) PCLP scaffold; (B) solvent casting scaffold.

Abbreviations: PCLP, powder mixing, compression molding, low-temperature treatment, and particulate leaching; SEM, scanning electron microscopy.

Figure 4 Results of in vitro tests: cell proliferation assessed by MTT method (A), ALP activity (B), and expression of osteogenic differentiation associated genes quantified by real-time RT-PCR (C, D, and E).

Notes: ^aP < 0.05; ^bP < 0.01; n = 3–6.

Abbreviations: ALP, alkaline phosphatases; MTT, methylthiazol tetrazolium.