Figures & data
Figure 1 Schematic representation for the synthesis of (A) hyaluronic acid-octadecanoic acid (HA-C18) and (B) alendronate-hyaluronic acid-octadecanoic acid (ALN-HA-C18).
![Figure 1 Schematic representation for the synthesis of (A) hyaluronic acid-octadecanoic acid (HA-C18) and (B) alendronate-hyaluronic acid-octadecanoic acid (ALN-HA-C18).](/cms/asset/ed6c489c-7912-4abd-b06e-318414133b77/dijn_a_12191027_f0001_b.jpg)
Table 1 The orthogonal design factors and levels
Figure 2 1H NMR spectrum of (A) hyaluronic acid (HA) and (B) alendronate-hyaluronic acid-octadecanoic acid (ALN-HA-C18).
![Figure 2 1H NMR spectrum of (A) hyaluronic acid (HA) and (B) alendronate-hyaluronic acid-octadecanoic acid (ALN-HA-C18).](/cms/asset/99b2c574-bc22-4836-ae64-3027bc0fe3b5/dijn_a_12191027_f0002_c.jpg)
Table 2 The orthogonal design L9 (34) and resulting values
Figure 4 The size distribution (A) and transmission electron microscopy (TEM) image (B) of CUR loaded ALN-HA-C18 micelles. (C) In vitro release characteristics of free CUR and CUR loaded ALN-HA-C18 micelles in 1% Tween 80 PBS medium. (D) Binding ratio of CUR, CUR loaded HA-C18 micelles, and CUR loaded ALN-HA-C18 micelles with hydroxyapatite. Data are presented as the mean ± SD (n=3). ***Significant difference between the two groups (***P<0.001).
![Figure 4 The size distribution (A) and transmission electron microscopy (TEM) image (B) of CUR loaded ALN-HA-C18 micelles. (C) In vitro release characteristics of free CUR and CUR loaded ALN-HA-C18 micelles in 1% Tween 80 PBS medium. (D) Binding ratio of CUR, CUR loaded HA-C18 micelles, and CUR loaded ALN-HA-C18 micelles with hydroxyapatite. Data are presented as the mean ± SD (n=3). ***Significant difference between the two groups (***P<0.001).](/cms/asset/f4e85e1d-b602-4b2d-a431-37bd81bd83c3/dijn_a_12191027_f0004_c.jpg)
Figure 5 In vitro cytotoxicity of blank ALN-HA-C18 micelles, free CUR, and CUR loaded ALN-HA-C18 micelles against HOB cells (A) and MG-63 osteosarcoma cells (B). Fluorescence microscope images (C) and flow cytometry analysis (D) of MG-63 cells incubated with free C6, C6 loaded ALN-HA-C18 micelles with HA and C6 loaded ALN-HA-C18 micelles. Data are presented as the mean ± SD (n=6). *, ** and *** represent P<0.05, P<0.01 and P<0.001, respectively. The bar is 50 μm.
![Figure 5 In vitro cytotoxicity of blank ALN-HA-C18 micelles, free CUR, and CUR loaded ALN-HA-C18 micelles against HOB cells (A) and MG-63 osteosarcoma cells (B). Fluorescence microscope images (C) and flow cytometry analysis (D) of MG-63 cells incubated with free C6, C6 loaded ALN-HA-C18 micelles with HA and C6 loaded ALN-HA-C18 micelles. Data are presented as the mean ± SD (n=6). *, ** and *** represent P<0.05, P<0.01 and P<0.001, respectively. The bar is 50 μm.](/cms/asset/5e2f3cc2-c3c6-40fd-9a3d-e50d54c7b1cf/dijn_a_12191027_f0005_b.jpg)
Figure 6 The in vivo antitumor activity of CUR loaded ALN-HA-C18 micelles on osteosarcoma bearing nude mice. (A) Tumor volume changes; and (B) body weight changes. (C) The hematoxylin and eosin (H&E) staining of the heart, liver, spleen, lung and kidney in saline and CUR loaded ALN-HA-C18 micelles group after treatment. Data are presented as the mean ± SD (n=6). *Represents P<0.05. Bars =100 μm.
![Figure 6 The in vivo antitumor activity of CUR loaded ALN-HA-C18 micelles on osteosarcoma bearing nude mice. (A) Tumor volume changes; and (B) body weight changes. (C) The hematoxylin and eosin (H&E) staining of the heart, liver, spleen, lung and kidney in saline and CUR loaded ALN-HA-C18 micelles group after treatment. Data are presented as the mean ± SD (n=6). *Represents P<0.05. Bars =100 μm.](/cms/asset/bc26781d-89a9-45ec-942a-e84261384985/dijn_a_12191027_f0006_c.jpg)